新材料,石油炼制,石油化工,精细化工,生物医药

分子光谱分析，纳米分析化学，生物分析化学

银离子;光学传感器;共振光散射;线性扫描;小牛胸腺DNA;核酸分子“光开关”;镉离子;L-丝氨酸;伏安法

Dynamic Light Scattering;DNA;Gold Nanoparticles;Triplex Dna;Fluorimetry;Detection Limit;Oligonucleotide;Fluorescence;Chemiluminescence;Absorption Spectroscopy

2013年 
1. Xiao, Z. Y., Guo, X. T., Ling, L. S.* Sequence-specific recognition of double-stranded DNA with molecular beacon with the aid of Ag+ under neutral pH environment. Chem. Commun., 2013, 49 (34), 3573 - 3575. 
2. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Sensitive detection of glucose in human serum with Oligonucleotide modified gold nanoparticles by using dynamic light scattering technique. Biosensor and Bioelectronics, 2013, 41, 880-883. 
3. Xiong, C., Ling, L.S.* Localized Surface Plasmon Resonance Light Scattering Sensor for Mercury(II) ion with Label-free Gold Nanoparticles. Journal of Nanoscience and Nanotechnology. 2013, 13, 1406-1410.
2012年 
4. Li, R.M., Xiong, C., Xiao, Z. Y., Ling, L.S.,* Colorimetric Detection of Cholesterol with G-Quadruplex-Based DNAzymes and ABTS2-. Analytica Chimica Acta. 2012, 724, 80-85. 
5. Xiong, C., Xiao, Z. Y., Zhang, M. J., Ling, L.S.* Sensitive detection of H2O2 and H2O2 - related reactant with Ru(bipy)2(7,8-dimethyl-dipyridophenazine)2+ and Oligodeoxyribonucleotide. Analyst, 2012, 137, 4428 – 4434. 
6. Miao, X. M., Ling, L.S.,* Cheng，D., Shuai, X.T.* Highly sensitive sensor for Cu2+ with unmodified gold nanoparticles and DNAzyme by using dynamic light scattering technique. Analyst, 2012, 137 (13), 3064 – 3069. 
7. Xiong, C., Ling, L.S.* Label-free, sensitive detection of Hg(II) with gold nanoparticles by using dynamic light scattering technique. Talanta, 2012, 317-321.  
8. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Detection of Pb2+ at attomole level by using dynamic light scattering and unmodified gold nanoparticles. Analytical Biochemistry, 2012，421, 582-586.  
2011年  
9. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Ultrasensitive detection of lead(II) with DNAzyme and gold nanoparticles probes by using a dynamic light scattering technique. Chem. Commun., 2011, 47 (14), 4192 – 4194.   
10. Miao, X. M., Xiong, C., Wang, W.W., Ling, L.S.,* Shuai, X.T.* Dynamic Light Scattering-Based Sequence Specific Recognition of Double Stranded DNA with Oligonucleotide Functionalized Gold Nanoparticles. Chemistry - A European Journal, 2011, 17, 11230-11236.   
11. Wu, C.K., Zhou, Y. M., Miao, X. M., Ling, L. S.* A novel fluorescent biosensor for sequence specific recognition of double-stranded DNA with the platform of graphene oxide. Analyst, 2011, 136 (10), 2106 – 2110.  
12. Xiong,C., Wu, C. K., Zhang, H., Ling, L.S.* Gold nanoparticles-based Colorimetric investigation of triplex formation under weak alkalic pH environment with the aid of Ag+. SPECTROCHIM  ACTA  A.,  2011, 79, 956–961.  
13. 吴呈珂，董秋鹏，张红，熊岑，吴秀丽，凌连生*, [Cu(DPPZ)(L-Ser)]+与DNA的相互作用及其分析应用研究. 光谱学与光谱分析，2011，31(4),1057-1060.   
2010年 
14. Wu C.K., Xiong, C., Wang, L., Lan, C., Ling, L.S.* Sensitive and Selective Localized Surface Plasmon Resonance Light-Scattering Sensor for Ag+ with Unmodified Gold Nanoparticles. Analyst, 2010, 135, 2682–2687.   
2009年 
15. Xin, A., Dong, Q. P., Xiong, C., Ling, L. S.* Colorimetric recognition of DNA intercalators with unmodified gold nanoparticles. Chem. Commun., 2009, 1658-1660.  
16. Yan, H. L., Xiong, C., Yuan, H., Zeng, Z. X., Ling, L.S.* Spacer control the dynamic of triplex formation between oligonucleotide modified gold nanoparticles. Journal of Physical Chemistry C. 2009, 113, 17326–17331.  
2008年以前 
17. Ling,L.S., Butt,H. J., Berger, R.* The rupture force between the third strand and double strand within a triplex DNA. J. Am. Chem. Soc. (Article) 2004, 126, 13992-13997.   
18. Ling,L.S., Butt,H. J., Berger, R.* Rupture Force Changes between the Third Strand and the Double Strand within a Triplex DNA in the Presence of Intercalative Molecules. Appl. Phys. Lett.. 2006, 89(11), 113902.   
19. Ling,L.S., Jiang, Y. X., Wang, C., Fang, X. H.*, Wan, L. J., He, Z. K., Bai, C. L.*, Chen, D. M.* Delaying Photobleaching and Recovering Luminescence of a Molecular Light Switch in DNA Analysis. Analytical Biochemistry, 2004, 329, 334-336.   
20. 凌连生, 汪俊，方晓红，陈东敏，白春礼*, 三螺旋脱氧核糖核酸的研究进展 (评述). 分析化学, 2004 32, 1252-1255.  
21. Yang, G. Z., Ling,L.S., Wan,L.J.*, Fang,X.H.,* Bai, C.L.* Electrochemical Scanning Tunneling Microscope Imaging of Self-Assembed Monolayer of Double-Stranded DNA on Au(111). Journal of Nanoscience and Nanotechnology, 2004, 4(6), 561-564.    
22. Li, B. S., Kang,S. Z., Cheuk,K.K.L., Wan, L.J., Ling,L.S. Bai,C.L.* Tang,B. Z.* Self-assembling of Helical Poly(Phenylacetylene) Carrying L-Valine Pendants in Solution, on Mica Substrate, and on Water Surface，Langmuir, 2004, 20, 7598-7603.   
23. Ling,L.S., He, Z. K.*, Chen, F., Zeng, Y. E. Single-mismatch detection using nucleic acid molecular "'light switch"'. Talanta, 2003, 59 (2), 269-275.   
24. Ling, L.S., Fang, X.H., Wang, C., Wan, L.J., Bai, C.L.* Chen, D.M., Direct observation of the DNA multimolecular condensation with fluorescence microscopy, Chemistry Letters, 2003, 32 (1), 80-81.   
25. Ling,L.S., He, Z. K.*, Chen, F., Zhang, H. S., Zeng, Y. E. Study on triplex DNA by use of molecular "Light Switch" complex of Ru(phen)2(dppx)2+. Chinese Chemical Letters. 2003, 14  
(3), 300-303.  
26. Jiang, Y.X., Zhu, C.F., Ling, L.S., Wan, L. J., Fang, X. H.*, Bai, C. L.* Specific Aptamer - Protein Interaction Studied by Atomic Force Microscopy, Analytical Chemistry, 2003, 75, 2112-2116.  
27. Li, B. S., Cheuk, K. K. L., Ling,L.S., Chen, J. W., Xiao, X. D., Bai, C. L.*, Tang, B. Z.* Synthesis and Hirarchical structures of amphilic polyphonylacetylenes carrying L-valine pendents. Macromolecules, 2003, 36, 77-85.  
28. Ling,L.S.*, He, Z. K., Song, G. W., Zeng, Y., Wang, C., Bai, C. L., Chen, X. D., Shen, P. High sensitive determination of DNA by use of molecular"Light Switch" complex of Ru(phen)2(dppx)2+ Analytica Chimica Acta.2001, 436(2), 207-214.   
29. Yang, J. L., Lin, H. Z., He, Q. G., Ling,L.S., Zhu, C. F., Bai, F. L.* Composition of hyperbranched conjugated polymers with nanosized cadium sulfide particles, Langmuir, 2001, 17(19), 5978-5983.   
30. 凌连生，何治柯*，曾云鹗, 核酸荧光探针的研究进展 (评述)，分析化学， 2001, 29 (6), 721-724.   
31. Ling,L.S., He, Z. K.*, Song, G. W., Zeng,Y. E. A novel method for determination of DNA by use of molecular "Light Switch" complex of Ru(bipy)2(dppx)2+ . Analytica Chimica Acta. 2000, 403 (1-2), 209-217.   
32. Ling,L.S., He, Z. K.*, Song, G. W., Zeng,Y. E. Determination of DNA by use of the molecular"Light Switch"complex of Ru(bipy)2(dppz)2+. Mikrochimica Aata 2000, 134(1-2), 57-62.   
33. 凌连生，何治柯*，吴凤武，罗庆尧，曾云鹗，核酸分子 "光开关" 的研究进展 (评述). 高等学校化学学报， 2000, 21 (4), 527-531.    
34. Li, X.Y., Ling,L.S., He, Z. K.*, Zeng,Y. E. Development of a Chemiluminescence method for the simultaneous determination of pyruvic and tartaric acids in human serum based upon their reaction with cerium(IV) in the presence of rutheniumtrispyridine. Microchemical Journal. 2000, 64, 9-13.   
35. Ling,L.S., He, Z. K.*, Song, G. W., Zeng,Y. E. Spectral studies on the interaction of Ru(phen)2(dppx)2+(phen=1,10-phenanthroline, dppx=7,8–dimethyl -dipyridophenazine) and DNA. SPECTROSCOPY LETT，1999, 32 (6), 931-939.   
36. Ling,L.S., Song, G. W., He, Z. K.*, Zeng,Y. E. A novel method to determine DNA by use of molecular "Light Switch" complex of Ru(phen)2(dppz)2+. Microchemical Journal. 1999, 63 (3), 356-364.     
37. Ling,L.S., He, Z. K.*, Zeng, Y. E. Spectral studies on the interaction of DNA and Ru(bipy)2(dppx)2+.SPECTROCHIM  ACTA  A. 1999, 55 (6), 1297-1302.   
38．He, Z. K.*, Wu, F. W., Meng, H., Ling,L.S., Zeng,Y. E. Chemiluminescence determination of thiorea using tris(2,2’-bipyridyl) ruthenium (II)-KMnO4 system. Analytical Sciences. 1999, 15 (4), 381-383.     
39. 何治柯*, 凌连生, 蔡汝秀,曾云鹗， Ru(2,2’-bipyridine)32+-α-羟基酸-Ce(IV) 化学发光反应机理研究，化学学报，1998, 56 (5), 472-477.   
40. 凌连生, 何治柯*, 蔡汝秀, Ru(bipy)32+-Ce(IV)-丙酮酸体系测定血清中的丙酮酸, 高等学校化学学报，1997,18(12),1963-1965.\

1. 一种检测前列腺特异性抗原的方法及其应用
中山大学
凌连生; 赵利珍; 胡玉玲
公开日期: 2024-03-26 申请日期: 2023-12-06 公开号: CN117757899A

2. 基于三螺旋DNA结合级联信号放大策略检测黄曲霉毒素B1的方法
中山大学
凌连生; 张曼君; 邹李
公开日期: 2023-10-31 申请日期: 2021-11-15 公开号: CN114184775B

3. 一种基于金纳米粒子和杂交链反应扩增检测甲醛的方法
中山大学
凌连生; 黄文秀; 赵利珍
公开日期: 2023-04-14 申请日期: 2022-12-19 公开号: CN115961011A

4. 一种基于金属纳米粒子和三螺旋链的甲醛比色检测方法及应用
中山大学
凌连生; 黄文秀
公开日期: 2022-02-11 申请日期: 2021-10-26 公开号: CN114034692A

5. 一种黄曲霉毒素m1的检测方法及其应用
SUN YAT-SEN UNIVERSITY
LING LIANSHENG; LI TINGTING; HUANG WENXIU
公开日期: 2021-08-10 申请日期: 2021-05-13 公开号: CN113234800A

6. 基于聚合酶链式反应和动态光散射检测血液中癌症标志物CA125的方法
中山大学
凌连生; 沈瑞迪; 黄文秀; 张继
公开日期: 2020-06-05 申请日期: 2020-01-22 公开号: CN111235228A

7. 基于杂交链式反应和动态光散射检测尿液中端粒酶活性的方法
中山大学
凌连生; 李婷婷; 邹李; 王京
公开日期: 2019-01-11 申请日期: 2018-09-18 公开号: CN109182456A

8. 基于杂交链式反应和动态光散射检测尿液中端粒酶活性的方法
中山大学
凌连生; 李婷婷; 邹李; 王京
公开日期: 2019-01-11 申请日期: 2018-09-18 公开号: CN109182456A

9. 一种实时动态光散射定量PCR仪
中山大学
凌连生; 邹李
公开日期: 2018-10-02 申请日期: 2018-04-02 公开号: CN108611267A

10. 一种基于金纳米颗粒的定量PCR检测方法
中山大学
凌连生; 邹李
公开日期: 2018-07-31 申请日期: 2018-04-25 公开号: CN108342459A

11. 一种基于金纳米颗粒的定量PCR检测方法
中山大学
凌连生; 邹李
公开日期: 2018-07-31 申请日期: 2018-04-25 公开号: CN108342459A

12. 一种多倍PCR扩增方法
中山大学
凌连生; 李海刚; 王京
公开日期: 2017-09-19 申请日期: 2017-07-19 公开号: CN107177693A

13. 一种多倍PCR扩增方法
中山大学
凌连生; 李海刚; 王京
公开日期: 2017-09-19 申请日期: 2017-07-19 公开号: CN107177693A

14. 一种基于自组装结构模拟酶及其制备方法与应用
中山大学
凌连生; 栗瑞敏
公开日期: 2016-07-27 申请日期: 2016-03-21 公开号: CN105798324A

15. 一种基于自组装结构模拟酶及其制备方法与应用
中山大学
凌连生; 栗瑞敏
公开日期: 2016-07-27 申请日期: 2016-03-21 公开号: CN105798324A

16. 一种钌(Ⅱ)多吡啶配合物的制备方法
武汉大学
何治柯; 凌连生; 黄珊; 吉邢虎
公开日期: 2007-06-27 申请日期: 2006-12-15 公开号: CN1986555A

作为负责人主持的科研项目：
1. 国家自然科学基金, 三螺旋DNA及其与蛋白质(酶)相互作用的纳米光学传感器的研究 (No:20975116).
2. 国家自然科学基金, 基于三螺旋DNA的纳米生物传感器的研究 (No: 20645002)
3. 教育部留学回国人员启动基金, 基于金纳米粒子的DNA纳米生物传感器的研究(2008.1-2009.12) 
4. 中山大学“百人计划”启动经费
5. 中国博士后科学基金的单个DNA分子凝聚的动力学研究(一等)
6. 中国科学院王宽诚博士后工作奖励基金
7. 北京分子科学国家实验室开放课题(2012.10-2014.9)

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