生物医药

真菌来源天然产物的特征环系结构的生物合成酶挖掘与新酶学机制解析，并通过人工生物合成途径改造设计，优化基因编辑工具以及开发新的底盘微生物细胞，研发出莫匹罗星、麦角生物碱、红曲红色素以及间甲酚的新发酵菌株，相关产品已经工业化并实现千吨级发酵

1, Jun Zhang, Daohong Liao, Rongchang Chen, Fangfang Zhu, Yaqing Ma, Lei Gao, Ge Qu, Chengsen Cui, Zhoutong Sun,* Xiaoguang Lei, * Shu-Shan Gao*, Tuning an Imine Reductase for the Asymmetric Synthesis of Azacycloalkylamines by Concise Structure-Guided Engineering. Angewandte Cheme International Edition, under revision.

2, Beyond the cyclopropyl ring formation: fungal Aj_EasH catalyzes asymmetric hydroxylation of ergot alkaloids. Chunyan An*, Fangfang Zhua, Yongpeng Yao, Kexin Zhang, Wei Wang, Jun Zhang, Guangzheng Wei, Yue Xia, Qiang Gao,*, Shu-Shan Gao,* Applied Microbiology and Biotechnology, 2022, in press.

3. Yongpeng Yao, Wei Wang, Wenyu Shi, Rui Yan, Jun Zhang, Guangzheng Wei, Ling Liu, Yongsheng Che, Chunyan An, Shu-Shan Gao*, Overproduction of medicinal ergot alkaloids based on a fungal platform, Metabolic Engineering, 2022, 69, 198-208 

4. Xian Shu, Guangzheng Wei, Yuben Qiao, Kexin Zhang, Jun Zhang, Guomin Ai, Man-cheng Tang, Yonghui Zhang, and Shu-Shan Gao*, TerC Is a Multifunctional and Promiscuous Flavoprotein Monooxygenase That Catalyzes Bimodal Oxidative Transformations. Org. Lett. 2021, 23, 22, 8947–8951 

5.  Guangzheng Wei,# Xian Shu,# Yongpeng Yao, Kexin Zhang, Jun Zhang, and Shu-Shan Gao*, Heterologous Production of Unnatural Flavipucine Family Products Provides Insights into Flavipucines Biosynthesis. Organic Letters, Org. Lett. 2021, 23, 20, 7708–7712 

6.  Wei Wang,# Chunyan An,# Yongpeng Yao, Xiangxi Meng, Shu-Shan Gao* De novo biosynthesis and gram-level production of m-cresol in Aspergillus nidulans. Applied Microbiology and Biotechnology, 2021, 105, 6333–6343. 

7. Weiwei Liu,# Chun-Yan An,# Xian Shu, Xiangxi Meng, Yongpeng Yao, Jun Zhang, Fusheng Chen, Hua Xiang, Shuyuan Yang, Xue Gao*, and Shu-Shan Gao.* A dual-plasmid CRISPR/Cas system for mycotoxin elimination in polykaryotic industrial fungi, ACS Synthetic Biology, 2020, 9, 8, 2087–2095. 

8. Yongpeng Yao,# Chunyan An,# Declan Evans, Weiwei Liu, Wei Wang, Guangzheng Wei, Ning Ding, K. N. Houk,* Shu-Shan Gao.* A catalase involved in the oxidative cyclization of the tetracyclic ergoline of fungal ergot alkaloids. Journal of the American Chemical Society 2019, 141, 44, 17517-17521. 

9. Mengbin Chen, Qikun Liu, Shu-Shan Gao, Abbegayle E. Young, Steven E. Jacobsen, and Yi Tang. Genome mining and biosynthesis of a polyketide from a biofertilizer fungus that can facilitate reductive iron assimilation in plant. PNAS, 2019, 116, 12, 5499-5504.

10. Tingting Hao, Zhoujie Xie, Min Wang, Liwei Liu, Yuwei Zhang, Weicang Wang, Zhao Zhang, Xuejin Zhao, Pengwei Li, Zhengyan Guo, Shushan Gao, Chunbo Lou, Guodong Zhang, Justin Merritt, Geoff P. Horsman & Yihua Chen*, An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters, Nature Communications 2019, 10, 1, 3665.