生物医药

糖合成生物学，创新非糖质资源依赖人工CO2-糖合成方法，开发灵活性、高效性及多功能性的人工CO2-糖合成平台；模拟自然寡糖和聚糖的合成途径，开发适应工业条件下的糖酶元件，构建体外生物转化系统以及微生物合成细胞工厂，创制具有特殊功能和应用场景的稀少糖、寡糖、多糖等食品配料产品，应用于未来食品等领域

1)  Wang Y.; Chen P.; Li W.; Wang Y.; Dong Q.; Song W.; Liu Y.; Cai T.; Sun Y.; Yang J*.; Ma Y. Cell-free synthesis of high-order carbohydrates from low-carbon molecules. Science Bulletin. 2025. 70, 2266–2276

2）Wang Y, Li W, Wang D, Zeng Y, Li M, Sun Y,Yang J*. Screening and characterization of thermostable xylose isomerase fromRhodothermus marinusfor erythrose production from one-carbon source.Enzyme Microb Tech.2025, 186, 110607    

3）Liu Y.; Dong Q.; Song W.; Pei W.; Zeng Y.; Wang M.; Sun Y.; Ma Y*.;Yang J*.Microbial synthesis of sedoheptulose from glucose by metabolically engineeredCorynebacterium glutamicum.Microbial Cell Factories. 2024, 23:251

4）Li, J., Li, R., Shang, N., Men, Y., Cai, Y., Zeng, Y., Liu, W., Yang, J*., Sun, Y*. Enzymatic synthesis of novel terpenoid glycoside derivatives decorated with N-acetylglucosamine catalyzed by UGT74AC1. J. Agric. Food Chem. 2024, 72, 25, 14255–14263

5）Wang Y.; Chen E.; Wang Y.; Sun X.; Dong Q.; Chen P.; Zhang C.; Yang J*.; Sun Y. Biosynthesis of mannose from glucose via constructing phosphorylation-dephosphorylation reactions in Escherichia coli. Enzyme Microb Tech. 2024, 177, 110427

6）Liu J.; Li J.; Chen P.; Zeng Y.; Yang J*.; Sun Y*. Efficient production of isomaltulose using engineered Yarrowia lipolytica strain facilitated by non-yeast signal peptide-mediated cell surface display. J. Sci. Food Agric, 2024, 104: 5999 – 6007

7）Yang, J†.; Song, W†.; Cai, T†.; Wang, Y.; Zhang, X.; Wang, W.; Chen, P.; Zeng, Y.; Li, C.; Sun, Y*.; Ma, Y*. De novo artificial synthesis of hexoses from carbon dioxide. Science Bulletin, 2023.68(20), 2370-2381

8）Sun, X#; Zhang, T#; Liu, Y; Chen, P; Qin, H;Yang, J*; Sun,Y. Self-assembled multienzyme complex facilitates synthesis of glucosylglycerol from maltodextrin and glycerol.J. Sci. Food Agric, 2024, 104(1):266-272

9）Zhang, T#.; Liu, P#.; Wei, H.; Sun, X.; Zeng, Y.; Zhang, X.; Cai, Y.; Cui, M.; Ma, H.;* Liu, W.;* Sun, Y.;*Yang, J*. Protein engineering of glucosylglycerol phosphorylase facilitating efficient and highly regio- and stereoselective glycosylation of polyols in a synthetic system.ACS Catalysis, 2022, 12, 24, 15715–15727.

10）Li,J#.;Mu,S#.; Yang, J*.; Liu, C.; Zhang,Y.;Chen,P.;Zeng,Y.; Zhu,Y.; Sun, Y*. Glycosyltransferase engineering and multi-glycosylation routes development facilitating synthesis of high-intensity sweetener mogrosides. iScience. 2022. 25(10), 105222

11）Tian, C#.;Yang, J#*.;Liu, C#.; Chen, P; Zhang, T.; Men, Y.; Ma, H*.; Sun, Y*.; Ma, Y. Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars. Nature Communication. 2022, 13, 3582.

12）Cai, T#.; Sun, H#.; Qiao, J#.; Zhu, L#.; Zhang, F.; Zhang, J.; Tang, Z.; Wei, X.; Yang, J.; Yuan, Q.; Wang, W.; Yang, X.; Chu, H.; Wang, Q.; You, C.; Ma, H.; Sun, Y.; Li, Y.; Li, C.; Jiang, H.; Wang, Q.; Ma, Y*.; Cell-free chemoenzymatic starch synthesis from carbon dioxide, Science, 2021, 372(6562): 1523-152 

13）Ren, C#.;Yang, J*.;Zeng, Y.; Zhang, T.; Tian, C.; Men, Y.; Sun, Y*.Novel catalytic property of fructose-6-phosphate aldolase in directly conversion of two 1-hydroxyalkanones to diketones. Enzyme and Microbial Technology, 2021, 147: 109784

14）Wang, W#.; Yang, J#.; Sun, Y*.; Li, Z*.; You, C*. Artificial ATP-free in vitro synthetic enzymatic biosystems facilitate aldolase-mediated C-C bond formation for biomanufacturing. ACS Catalysis, 2020, 10(2):1264-1271

15）Tian, C#.; Yang, J#*.; Li, Y#.; Zhang, T.; Li, J.; Ren, C.; Men, Y.; Chen, P.; You, C*.; Sun, Y*.; Ma, Y. Artificially designed routes for the conversion of starch to value-added mannosyl compounds through couplingin vitroandin vivometabolic engineering strategies. Metabolic Engineering, 2020, 61:215-224