生物医药

极端微生物合成生物学、极端环境微生物组、CRISPR-Cas分子机制、基因组编辑技术创新等

[1] Guo J,Gong L,Yu H,Li M,An Q,Liu Z,Fan S,Yang C,Zhao D,Han J,Xiang H*. Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells. Nature Communications. 2024;15(1):7277. Doi:10.1038/s41467-024-51695-x
[2] Zhao D,Zhang S,Chen J,Zhao J,An P,Xiang H*. Members of the class Candidatus Ordosarchaeia imply an alternative evolutionary scenario from methanogens to haloarchaea. The ISME Journal. 2024;18(1). Doi:10.1093/ismejo/wrad033
[3] Liu C,Wang R,Li J,Cheng F,Shu X,Zhao H,Xue Q,Yu H,Wu A,Wang L,Hu S,Zhang Y,Yang J,Xiang H*,Li M*. Widespread RNA-based cas regulation monitors crRNA abundance and anti-CRISPR proteins. Cell Host & Microbe. 2023;31(9):1481-93 e6. Doi:10.1016/j.chom.2023.08.005
[4] Cheng F,Wu A,Liu C,Cao X,Wang R,Shu X,Wang L,Zhang Y,Xiang H*,Li M*. The toxin-antitoxin RNA guards of CRISPR-Cas evolved high specificity through repeat degeneration. Nucleic Acids Research. 2022;50(16):9442-52. Doi:10.1093/nar/gkac712
[5] Xu T,Chen J,Mitra R,Lin L,Xie Z,Chen GQ,Xiang H*,Han J*. Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation. Communications Biology. 2022;5(1):623. Doi:10.1038/s42003-022-03570-y
[6] Li M,Gong L,Cheng F,Yu H,Zhao D,Wang R,Wang T,Zhang S,Zhou J,Shmakov SA,Koonin EV,Xiang H*. Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems. Science. 2021;372(6541). Doi:10.1126/science.abe5601
[7] Cheng F,Wang R,Yu H,Liu C,Yang J,Xiang H*,Li M*. Divergent degeneration of creA antitoxin genes from minimal CRISPRs and the convergent strategy of tRNA-sequestering CreT toxins. Nucleic Acids Research. 2021;49(18):10677-88. Doi:10.1093/nar/gkab821
[8] Gong L,Li M,Cheng F,Zhao D,Chen Y,Xiang H*. Primed adaptation tolerates extensive structural and size variations of the CRISPR RNA guide in Haloarcula hispanica. Nucleic Acids Research. 2019;47(11):5880-91. Doi:10.1093/nar/gkz244
[9] Xu Z,Li M,Li Y,Cao H,Miao L,Xu Z,Higuchi Y,Yamasaki S,Nishino K,Woo PCY,Xiang H*,Yan A*. Native CRISPR-Cas-mediated genome editing enables dissecting and sensitizing clinical multidrug-resistant P. aeruginosa. Cell Reports. 2019;29(6):1707-17 e3. Doi:10.1016/j.celrep.2019.10.006
[10] Li M,Gong L,Zhao D,Zhou J,Xiang H*. The spacer size of I-B CRISPR is modulated by the terminal sequence of the protospacer. Nucleic Acids Research. 2017;45(8):4642-54. Doi:10.1093/nar/gkx229
[11] Wang R,Li M,Gong L,Hu S,Xiang H*. DNA motifs determining the accuracy of repeat duplication during CRISPR adaptation in Haloarcula hispanica. Nucleic Acids Research. 2016;44(9):4266-77. Doi:10.1093/nar/gkw260
[12] Yang H,Wu Z,Liu J,Liu X,Wang L,Cai S,Xiang H*. Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins. Nature Communications. 2015;6:8321. Doi:10.1038/ncomms9321
[13] Wu Z,Liu J,Yang H,Liu H,Xiang H*. Multiple replication origins with diverse control mechanisms in Haloarcula hispanica. Nucleic Acids Research. 2014;42(4):2282-94. Doi:10.1093/nar/gkt1214
[14] Li M,Wang R,Zhao D,Xiang H*. Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process. Nucleic Acids Research. 2014;42(4):2483-92. Doi:10.1093/nar/gkt1154
[15] Li M,Wang R,Xiang H*. Haloarcula hispanica CRISPR authenticates PAM of a target sequence to prime discriminative adaptation. Nucleic Acids Research. 2014;42(11):7226-35. Doi:10.1093/nar/gku389