生物医药

1. 建立重要氨基酸工业底盘谷氨酸棒杆菌的高效基因组编辑和精细表达调控技术
2. 挖掘改造氨基酸合成关键功能元件
3. 设计创制自主、高性能氨基酸工业菌种

1. Liu J, Liu M, Shi T, Sun G, Gao N, Zhao X, Guo X, Ni X, Yuan Q, Feng J, et al. CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction. Nature Communications. 2022;13:891.
2. Liu J, Wang Y, Lu Y, Ni X, Guo X, Zhao J, Chen J, Dele-Osibanjo T, Zheng P, Sun J, Ma Y. Mutations in Peptidoglycan Synthesis Gene ponA Improve Electrotransformation Efficiency of Corynebacterium glutamicum ATCC 13869. Appl Environ Microbiol. 2018;84.
3. Liu J, Wang Y, Zheng P, Sun J. CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum. Bio Protoc. 2018;8:e3038.
4. Liu J, Wang Y, Lu Y, Zheng P, Sun J, Ma Y. Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum. Microb Cell Fact. 2017;16:205.
5. Wang XC, Liu J, Zhao J, Ni XM, Zheng P, Guo X, Sun CM, Sun JB, Ma YH. Efficient production of trans-4-hydroxy-l-proline from glucose using a new trans-proline 4-hydroxylase in Escherichia coli. J Biosci Bioeng. 2018;126:470-477.
6. Hu X, Huang X, Liu J, Zheng P, Gong W, Yang L. Structures of L-proline trans-hydroxylase reveal the catalytic specificity and provide deeper insight into AKG-dependent hydroxylation. Acta Crystallogr D Struct Biol. 2023;79:318-325.
7. Wang Y, Liu Y, Liu J, Guo Y, Fan L, Ni X, Zheng X, Wang M, Zheng P, Sun J, Ma Y.MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. Metab Eng. 2018;47:200-210.