生物医药

新型菌种进化和高通量筛选技术方法的开发，主持或参与了多项氨基酸及衍生物高效菌种的从头创制和迭代提升工作，在氨基酸及其衍生物高效合成技术开发及产业化应用方面具有丰富的经验

Cai NY#, Chen JZ#, Gao N, Ni XM, Lei Y, Pu W, Wang LX, Che B, Fan LW, Zhou WJ, Feng JH, Wang Y, Zheng P, Sun JB. Engineering of the DNA replication and repair machinery to develop binary mutators for rapid genome evolution of Corynebacterium glutamicum. Nucleic Acids Res. 2023, 51: 8623-8642.
Pu W#, Chen JZ#, Liu P#, Shen J, Cai NY, Liu BY, Lei Y, Wang LX, Ni XM, Zhang J, Liu J, Zhou YY, Zhou WJ, Ma HW, Wang Y, Zheng P, Sun JB. Directed evolution of linker helix as an efficient strategy for engineering LysR-type transcriptional regulators as whole-cell biosensors. Biosens Bioelectron. 2023, 222: 115004.
Pu W#, Chen JZ#, Zhou YY, Qiu HM, Shi T, Zhou WJ, Guo X, Cai NY, Tan ZJ, Liu J, Feng JH, Wang Y, Zheng P, Sun JB. Systems metabolic engineering of Escherichia coli for hyper-production of 5-aminolevulinic acid. Biotechnol Biof Biop. 2023, 16: 31.
Zhou YY#, Chen JZ#, Pu W, Cai NY, Che B, Yang JX, Wang MM, Zhong SS, Zuo XT, Wang DP, Wang Y, Zheng P, Sun JB. Development of a growth-coupled selection platform for directed evolution of heme biosynthetic enzymes in Corynebacterium glutamicum. Front Bioeng Biotechnol. 2023, 11: 1236118.
Chen JZ, Wang Y, Zheng P, Sun JB. Engineering synthetic auxotrophs for growth-coupled directed protein evolution. Trends Biotechnol. 2022, 40: 773-776.
Huang JW#, Chen JZ#, Wang Y, Shi T, Ni XM, Pu W, Liu J, Zhou YY, Cai NY, Han SY, Zheng P, Sun JB. Development of a hyperosmotic stress inducible gene expression system by engineering the MtrA/MtrB-dependentNCgl1418promoter inCorynebacterium glutamicum. Front. Microbiol. 2021, 12: 718511.
Chen JZ#, Wang Y#, Guo X, Rao DM, Zhou WJ, Zheng P, Sun JB, Ma YH. Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered Corynebacterium glutamicum. Biotechnol Biofuels. 2020, 13: 41.
Li MY#, Chen JZ#, Wang Y, Liu J, Huang JW, Chen N, Zheng P, Sun JB. Efficient multiplex gene repression by CRISPR-dCpf1 inCorynebacterium glutamicum.Front Bioeng Biotechnol. 2020, 8: 357.
Zhu CC#, Chen JZ#, Wang Y, Wang LX, Guo X, Chen N, Zheng P, Sun JB, Ma Y. Enhancing 5-aminolevulinic acid tolerance and production by engineering the antioxidant defense system of Escherichia coli. Biotechnol Bioeng. 2019, 116: 2018-2028.
Sun DH#, Chen JZ#, Wang Y, Li MY, Rao DM, Guo YM, Chen N, Zheng P, Sun JB, Ma YH. Metabolic engineering of Corynebacterium glutamicum by synthetic small regulatory RNAs. J Ind Microbiol Biotechnol. 2019; 46: 203-208.
Zhang LL#, Chen JZ#, Chen N, Sun JB, Zheng P, Ma YH, Cloning of two 5-aminolevulinic acid synthase isozymes HemA and HemO fromRhodopseudomonas palustriswith favorable characteristics for 5-aminolevulinic acid production, Biotechnol Lett. 2013, 35: 763-768.
Chen JZ, Tao GJ, Wang XY, Construction of an Escherichia coli mutant producing monophosphoryl lipid A, Biotechnol Lett. 2011, 33: 1013-1019.
李学朋, 陈久洲*, 张东旭, 李树标, 王小平, 吕金东, 周敬, 许志颖, 郑平, 孙际宾. L-谷氨酸生产关键技术创新与产业化应用. 生物工程学报. 2022, 38(11):4343-4351.
陈久洲, 王钰, 蒲伟, 郑平, 孙际宾. 5-氨基乙酰丙酸生物合成技术的发展及展望. 合成生物学, 2021, 2(6): 1000-1016.
陈久洲, 李烨, 王小元. 细菌类脂A的结构修饰及其应用前景, 微生物学通报, 2010, 37(11):1685-1691.

郑平, 陈久洲, 蔡柠匀, 孙际宾, 周文娟, 王钰. 一种突变体库、其包含的突变体及在菌株进化中的应用. 202310311874.8.
孙际宾, 陈久洲, 李德衡, 石拓, 郑平, 蔡柠匀, 李广玉, 周文娟, 赵兰坤, 李树标. 一种氨基酸高产菌株的构建方法及其应用. 202310776132.2
孙际宾, 陈久洲, 蒲伟, 郑平, 周文娟, 石拓, 蔡柠匀. 高产5-氨基乙酰丙酸的工程菌株及5-氨基乙酰丙酸高产菌株的构建方法. 202210940821.8.
郑平, 陈久洲，孙际宾，蔡柠匀，郭轩，周文娟，刘岯，刘娇，马延和. 丙酮酸脱氢酶的突变体及其用于生产L-氨基酸的方法, ZL202110794028.7/WO2022094731A1
郑平, 陈久洲, 黄婧文, 孙际宾, 周文娟, 王钰, 马延和. 具有启动子活性的多核苷酸及其用途和生产目标化合物的方法，202110504946.1/WO2022152036A1.
郑平, 陈久洲, 黄婧文, 孙际宾, 周文娟, 石拓, 马延和. 一种突变的高渗诱导型启动子PproP及其应用.ZL202110841507.X.
孙际宾, 陈久洲, 郑平, 饶德明, 王钰, 周文娟, 郭轩, 马延和. 5-氨基乙酰丙酸生产菌株及其构建方法和应用.ZL202010059928.2.
孙际宾, 陈久洲, 郑平,周文娟, 郭轩, 马延和. 生产赖氨酸的微生物以及赖氨酸的生产方法. 中国,ZL202010042203.2/WO2021071517A1.
孙际宾, 陈久洲, 郑平, 朱成超, 郭轩, 周文娟, 马延和. 5-氨基乙酰丙酸合成酶突变体及其宿主细胞和应用.ZL201910425976.6.
郑平, 陈久洲, 蒲伟, 孙际宾, 马延和. 5-氨基乙酰丙酸高产菌株及其制备方法和应用. 中国, ZL201310051018.X/WO2014071712A1.

技术咨询