生物医药

1. 枯草芽孢杆菌蛋白质表达系统的建立、优化及多种高附加值蛋白质产品的高效表达
2. B族、K族维生素的生物合成技术开发

Jinlong Li, Sijia Wang, Cui Liu, Yixin Li, Yu Wei, Gang Fu, Pi Liu, Hongwu Ma, Dawei Huang, Jianping Lin* and Dawei Zhang*. Going beyond the local catalytic activity space of chitinase using a simulation-based iterative saturation mutagenesis strategy. ACS Catalysis. 2022 Aug 5. 12, 16, 10235–10244
Fu, G.; Yue, J.; Li, D.; Li, Y.; Lee, S. Y.; Zhang, D., An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation. Proc Natl Acad Sci U S A 2022, 119 (11), e2119980119.
Yuan, M.; Fu, G.; Sun, Y. M.; Zhang, D. W., Biosynthesis and applications of curdlan. Carbohyd Polym 2021, 273.
Zhao, L. Q.; Cui, Y. Y.; Fu, G.; Xu, Z. X.; Liao, X. P.; Zhang, D. W., Signal Recognition Particle Suppressor Screening Reveals the Regulation of Membrane Protein Targeting by the Translation Rate. Mbio 2021, 12 (1).
Wang, S.; Fu, G.; Li, J.; Wei, X.; Fang, H.; Huang, D.; Lin, J.; Zhang, D., High-Efficiency Secretion and Directed Evolution of Chitinase BcChiA1 in Bacillus subtilis for the Conversion of Chitinaceous Wastes into Chitooligosaccharides. Front Bioeng Biotechnol 2020, 8, 432.
Gang Fu, Shibin Zhang, Huina Dong, Jingqi Chen, Ran Tu, Dawei Zhang, Enhanced production of d-psicose 3-epimerase in Bacillus subtilis by regulation of segmented fermentation. Biotechnol Appl Biochem. 2020 Sep;67(5):812-818.
Li, D.#; Fu, G.#; Tu, R.; Jin, Z.; Zhang, D., High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components. Microbial cell factories 2019, 18 (1), 17.
Fu, G.; Liu, J.; Li, J.; Zhu, B. W.; Zhang, D., Systematic screening of optimal signal peptides for secretory production of heterologous proteins in Bacillus subtilis. Journal of agricultural and food chemistry 2018, 66 (50), 13141-13151.