生物医药

在放线菌底盘内开发及应用基因组编辑、基于生物传感器和液滴微流控的高通量筛选等相关使能技术，解决底层关键共性技术难题；同时，在放线菌底盘内进行天然产物挖掘和高效生物合成机制解析，实现高附加值天然产物的生物制造及工程菌株的高通量智能铸造。

Yue Zhang*, Xuemei Zhang, Meng Wang*. Product-driven high-throughput screening of industrial filamentous actinomycetes. Trends in Biotechnology, 2023, 41(9): 1109-1112.
Jin Wang, Ning Xue, Wenjia Pan, Ran Tu, Shixin Li, Yue Zhang, Yufeng Mao, Ye Liu, Haijiao Cheng, Yanmei Guo, Wei Yuan, Xiaomeng Ni, Meng Wang*. Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids. Nature Communications, 2023, 14(1): 6680.
Yan Wang, Shixin Li, Ning Xue, Lixian Wang, Xuemei Zhang, Longqian Zhao, Yanmei Guo, Yue Zhang*, Meng Wang*. Modulating sensitivity of an erythromycin biosensor for precise high-throughput screening of strains with different characteristics. ACS Synthetic Biology, 2023, 12(6): 1761-1771.
Xuemei Zhang, Yan Wang, Yue Zhang*, Meng Wang*. CRISPR/Cas9-Mediated Multi-Locus Promoter Engineering in ery Cluster to Improve Erythromycin Production in Saccharopolyspora erythraea. Microorganisms, 2023, 11: 623.
Erbing Hua#, Yue Zhang#*, Kaiyue Yun, Wenjia Pan, Ye Liu, Shixin Li, Yan Wang, Ran Tu*, Meng Wang*. Whole-cell biosensor and producer co-cultivation-based microfludic platform for screening Saccharopolyspora erythraea with hyper erythromycin production. ACS Synthetic Biology, 2022, 11(8): 2697-2708.（封面文章）
Kaiyue Yun#, Yue Zhang#, Shixin Li, Yan Wang, Ran Tu, Hao Liu*, Meng Wang*. Droplet-Microfluidic-Based Promoter Engineering and Expression Fine-Tuning for Improved Erythromycin Production in Saccharopolyspora erythraea NRRL 23338. Frontiers in Bioengineering and Biotechnology, 2022, 10: 864977.
Yue Zhang, Kaiyue Yun, Huamei Huang, Ran Tu, Erbing Hua, Meng Wang*. Antisense RNA interference-enhanced CRISPR/Cas9 base editing method for improving base editing efficiency in Streptomyces lividans 66. ACS Synthetic Biology, 2021, 10(5): 1053-1063.（封面文章）
Ran Tu#, Yue Zhang#, Erbing Hua, Likuan Bai, Huamei Huang, Kaiyue Yun, Meng Wang*. Droplet-based microfluidic platform for high-throughput screening of Streptomyces. Communications Biology, 2021, 4: 647.
Xiao Wen, Yue Zhang, Haijiao Cheng, Jingjing An, Yanmei Guo, Lixian Wang, Meng Wang. A CRISPR/dCas9-assisted system to clone toxic genes in Escherichia coli. Biochimica et Biophysica Acta (BBA) - General Subjects, 2021, 1865(11): 129994.
Chun-Yan Lin#, Yue Zhang#, Ji-Hua Wu, Rong-Hui Xie, Jianjun Qiao, Guang-Rong Zhao*. Regulatory Patterns of Crp on Monensin Biosynthesis in Streptomyces cinnamonensis. Microorganisms, 2020, 8(2): 271.
Chun-Yan Lin, Ai-Ping Pang, Yue Zhang, Jianjun Qiao, Guang-Rong Zhao*. Comparative Transcriptomic Analysis Reveals the Significant Pleiotropic Regulatory Effects of LmbU on Lincomycin Biosynthesis. Microbial Cell Factories, 2020, 19(1): 30.
Han Xiao#*, Yue Zhang#, Meng Wang*. Discovery and engineering of cytochrome P450s for terpenoid biosynthesis. Trends in Biotechnology, 2019, 37(6): 618-631.