环保,生物医药

1. 生物分离新技术的开发：分子印迹技术、涡轮连续逆流高效节能萃取技术、两水相技术、层析新技术研究、色谱分离行为研究与色谱模拟等；
2. 生物产品的分离纯化研究：各种生物产品分离纯化技术的开发，如应用膜分离和层析技术进行产品环保新技术开发，抗体、酶、有机酸和抗生素等的分离纯化新工艺研究；不同饱和度化合物纯化、产品传统工艺改进等；
3. 生物产品分析检测及其杂质分析。

1. Guo Y., Chen X., Fang G., Cao X., Wan J., A convenient strategy for studying antibody aggregation and inhibition of aggregation: characterization and simulation. Pharmaceutics, 2025, 17, 534.
2. Zheng C., Guo Y., Zhang C., Cao X., Wan J., Nitrogen-defective protonated porous C3N4 nanosheets for enhanced photocatalytic hydrogen production under visible light，Applied Catalysis B: Environment and Energy，2025, 365：124879.
3. Zheng C., Lu H., Guo Y., Cao X., Wan J., g-C3N4 nanorods modified with N defects via the molten salt method: efficient photocatalysts for hydrogen production†, Catalysis Science & Technology, 2025, 15:3753-376.
4. Zheng X., Zheng T., Wang W., Cao X., Wan J., Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system, Preparative biochemistry & biotechnology, 2005, 1-15.
5. Yu X., Hu Y., Cao Z., Yan M., Xin J., Zheng S., Wan J., Cao X., Computational design and preparation of water-compatible noncovalent imprinted microspheres. Journal of Chromatography A. 2024, 1725 (464876).
6. Yu X., Chen H., Sha Z., Hu Y., Yan M., Xin J., Cao X., Wan J., Highly selective separation and purification of lincomycin by macroporous adsorption resin column chromatography. Journal of Chromatography A. 2024, 1735 (465282).
7. Guo Y., Chen X., Yu X., Wan J., Cao X., Prediction and validation of monoclonal antibodies separation in aqueous two-phase system using molecular dynamic simulation. Journal of Chromatography A. 2023, 1694: 463921.
8. Yu X., Liao J., Zeng H., Wan J., Cao X., Synthesis of water-compatible noncovalent imprinted microspheres for acidic or basic biomolecules designed based on molecular dynamics. Polymer. 2022: 125253.
9. Chen X., Guo Y., Yang T., Wan J., Cao X., Separation of antibody IgG201 by an aqueous two-phase system with recyclable pH-responsive polymers. Process Biochemistry. 2022: 125-133.
10. Chen X., Wei Y., Yang T., Guo Y., Wan J., Cao X., Separation of recombinant monoclonal antibodies IgG201 from a cell culture supernatant using an integrated aqueous two-phase system with thermo-separating EOPO. Separation and Purification Technology. 2021, 275: 119246.
11. Zeng H., Yu X., Wan J., Cao X., Synthesis of molecularly imprinted polymers based on boronate affinity for diol-containing macrolide antibiotics with hydrophobicity-balanced and pH-responsive cavities. Journal of Chromatography A. 2021: 461969.
12. Yu X., Zeng H., Wan J., Cao X., Computational design of a molecularly imprinted polymer compatible with an aqueous environment for solid phase extraction of chenodeoxycholic acid. Journal of Chromatography A. 2020, 1609: 460–490.
13. Dong L. Wan J., Cao X., Separation of transglutaminase using aqueous two-phase systems composed of two pH-response polymers. Journal of Chromatography A. 2018, 1555: 106–112.
14. Wan J., Dong W., Cao X., Polymerization of a new thermo-responsive copolymer with N-vinylcaprolactam and its application in recyclable aqueous two-phase systems with another thermo-responsive polymer. Bioresource and Bioprocessing. 2018, 5: 44-49.
15. Yu T., Lin M., Wan J., Cao X., Molecular interaction mechanisms in reverse micellar extraction of microbial transglutaminase. Journal of Chromatography A. 2017, 1511: 25-36.
16. Xu C., Wan J., Cao X., Synthesis of thermo-responsive polymers recycling aqueous two-phase systems and phase formation mechanism with partition of ε-polylysine. Journal of Chromatography A. 2016, 1472: 44–54.
17. Wan J., Li Q., Zhou F., Liu J., Cao X., Influence of chaotropes on recovery of trypsin and micellar sizes during reverse micelle extraction. Separation and Purification Technology. 2013, 116: 307-312.
18. Wan J., He J., Cao X., A novel process for preparing pure chenodeoxycholic acid from poultry Bile. Journal of Industrial and Engineering Chemistry. 2012, 18: 65-71.
19. Shuang L., Wan J., Zhu J., Cao X., Effects of porogens on the morphology and enantioselectivity of core-shell molecularly imprinted polymers with ursodeoxycholic acid. Separation and Purification Technology. 2010, 72: 208–216.
20. Wan J., Wang W., Biao N., Cao X., Partition of Several bioproducts in a recycling aqueous two-phase system with pH-light responsive copolymers. Separation and Purification Technology. 2010, 76: 104-109.

1. 国家自然科学基金，再生型两水相体系成相与分配的分子调控机制；
2. 国家自然科学基金，促溶离子提高反胶束萃取效率的机制与应用研究；
3. 国家自然科学基金，多孔硅胶表面分子印迹吸附剂在超临界条件下的分离行为；
4. 国家重点研发项目子课题，油酸及壬二酸的分离技术开发；
5. 国家重点研发项目子课题，生物质PX合成前体分离纯化技术开发；
6. 校企合作，涡轮萃取技术在抗生素分离中的应用研究；
7. 校企合作，发酵液中色素的分析、鉴定与脱色研究；
8. 校业合作，林可霉素原料药分离工艺开发与杂质去除研究；
9. 校企合作，涡轮萃取技术在有机酸分离中的应用研究；
10.校企合作，抗体类药物高效低成本ProteinA替代技术开发。