精细化工,生物医药

生物化工，发酵工程，代谢工程，微生物生理和代谢调控，合成生物学，具体包括：
1. 平台化合物和生物活性物质的生物制造和体外生物合成技术。
2. 非粮废弃资源（乙酸、CO2等）循环高效利用。
3. 常规和基因工程微生物的代谢调控、发酵过程优化、放大及与培养过程相适应的生物反应器工程研究。

1. Wang W, Yang JG, Sun YX*, Li ZM*, You C*. 2020. Novel ATP-free in vitro Synthetic Enzymatic Biosystems Facilitate Asymmetric C-C Bond Formation for Biomanufacturing. ACS Catalysis. 12: 1264-1271.
2. Liu H, Hou YH, Wang Y, Li ZM*. 2020. Enhancement of sulfur conversion rate in the production of L-cysteine by engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 68, 1: 250-257.
3. Zhang X, Cui XW, Shen S, Li ZM*. 2020. Engineering of supramolecular nanoreactors by assembly of multiple enzymes for ATP regeneration in vitro. Biochemical Engineering Journal. 155: 107487.
4. Zhang X, Cui XW, Li ZM*. 2020. Characterization of Two Polyphosphate Kinase 2 Enzymes 1 Used for ATP Synthesis. Applied Biochemistry and Biotechnology,
5. Yang K, Li ZM*. 2020. Multistep construction of metabolically engineered Escherichia coli for enhanced cytidine biosynthesis. Biochemical Engineering Journal. 154: 107433.
6. Huang B, Fang GC, Sun JB,, Wu H, Li ZM*, Ye Q. 2019. Efficient Biosynthesis of Succinate from Paper Mill Wastewater by Engineered Escherichia coli. Applied Biochemistry and Biotechnology.
7. Wang W, Meng DD, Li Q, Li ZM*, You C. 2019. Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem. Bioresour. Bioprocess., 6:
8. Shen S, Zhang X, Li ZM*. 2019. Development of an engineered carbamoyl phosphate synthetase with released sensitivity to feedback inhibition by site-directed mutation and casting error-prone PCR. Enzyme and Microbial Technology, 129.
9. Cui XW, Wan JX, Zhang X, Wu H, Huang B, Li ZM*, Ye Q. 2019. Efficient glutathione production in metabolically engineered Escherichia coli strains using constitutive promoters. Journal of Biotechnology. 289: 39-45.
10.  Jiang JQ, Huang B, Wu H*, Li ZM*, Ye Q. 2018. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresour. Bioprocess., 5:34-43.
11. Liu H, Fang GC, Wu H*, Li ZM*, Ye Q. 2018. L-Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy. Biotechnol. J, 13, 1700695.
12. Huang B, Yang H, Fang GC, Zhang X, Wu H*, Li ZM*, Ye Q. 2018. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering, 115: 943-954.
13.  Wang W, Liu MX, You C, Li ZM*, Zhang YHP*. 2017. ATP-free biosynthesis of a high-energy phosphate metabolite fructose 1,6-diphosphate by in vitro metabolic engineering. Metabolic Engineering 42: 168–174.
14. Wang RW, Yang J, Zhang GQ, Chao YP*, Li ZM*, Ye Q, Qian SJ. 2017. Co-expression of Beta-Glucosidase and Laccase in Trichoderma reesei by Random Insertion with Enhanced Filter Paper Activity. Molecular Biotechnology. 59(8): 353-364.
15. Zhang X, H Wu, Huang B, ZM Li*, Q Ye. 2017. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 214:63-68.
16. Cheng Z, Jiang JQ, Wu H, Li ZM*, Ye Q. 2016. Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. Bioresource Technology. 200: 897-904.
17. Kou FY, J Zhao, J Liu, J Shen, Ye Q, P Zheng, Li ZM*, JB Sun*, YH Ma. 2016. Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH. DOI: 10.1016/j.molcatb.2016.11.023
18. Li YJ, Huang B, Wu H*, Li ZM*, Ye Q and Zhang Y-HP. 2016. Production of Succinate from Acetate by Metabolically Engineered Escherichia coli. ACS Synthetic Biology. DOI: 10.1021/acssynbio.6b00052.
19. Yang JH, Li W, Wang DZ, Wu H*, Li ZM*, Ye Q. 2016. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 100: 6279-6289.
20. Zhang J, Quan C, Wang C, Wu H*, Li ZM*, Ye Q. 2016. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 15:38.
21. Wang DZ, Wang C, Wu H*, Li ZM*, Ye Q. 2016. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 43:45-53.
22. Huang YN, Li ZM* and Ye Q*. 2016. Transcriptional Regulation of Genes Involved in 3-Hydroxypropionic Acid Production in Response to Aeration of Recombinant Klebsiella pneumoniae. Applied Biochemistry and Biotechnology, 2016,178: 1129–1140.
23. Ye Q*, Li ZM, Wu H. 2016. Principle and performance of gas self-inducing reactors and applications to biotechnology. Advances in Biochemical Engineering/Biotechnology. 152:1-33.
24. Wang C, Zhang J, Wu H*, Li ZM*, Ye Q. 2015. Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 214:63-68.

生物合成谷胱甘肽研究
大肠杆菌代谢工程研究
多酶组合型催化体系研究
谷氨酸棒杆菌代谢工程研究
酿酒酵母代谢工程研究
微生物发酵优化放大