生物医药

1．微生物代谢工程合成平台化合物和生物活性物质
2．合成生物学中“代谢晶体管”研究及其应用
3．益生菌和后生元功能性食品关键技术研发

1. Peng Fei#, Wenrui Zhang#, Yanzhe Shang#, Peng Hu, Yang Gu, Yuanchan Luo*, Hui Wu*. Carbon-negative bio-production of short-chain carboxylic acids (SCCAs) from syngas via the sequential two-stage bioprocess by Moorella thermoacetica and metabolically engineered Escherichia coli. Bioresource Technology. 2025. 416:131714 (JCR Q1)
2. Xinting Jiang, Xiaomin Chen, Hongxia Gao, Jinyan Luo, Lin Zhang, Yuanchan Luo*, Hui Wu*. Impact of energy metabolism pathways in promoting phytoremediation of cadmium contamination by Bacillus amyloliquefaciens Bam1. Bioresources and Bioprocessing. 2025. 12:134. (JCR Q1)
3. Wenrui Zhang#, Hongjun Zhang#, Yanzhe Shang, Yuanchan Luo, Haizhen Wu, Hui Wu*. High-yield biosynthesis of 3-hydroxypropionic acid from acetate in metabolically engineered Escherichia coli. ACS Synthetic Biology. 2025. 14:1654–1666. (JCR Q1)
4. Ju Wu, Xuan Gong, Pengye Guo, Yanzhe Shang, Yuanchan Luo, Hui Wu*. Biosynthesis of poly(3-hydroxybutyrate-co-lactate) using an NADH regeneration strategy in E. coli. BioDesign Research. 2025. 7(3):100027. (JCR Q1)
5. Jing Peng#, Junru Sun#, Yuanchan Luo, Hui Wu*. Evolution-assisted engineering of E. coli improved succinic acid production from glycerol. BioDesign Research. 2025. 7(1):100022. (JCR Q1)
6. Yanzhe Shang#, Zhengtong Zhu#, Junru Sun, Peng Fei, Yuanchan Luo, Hui Wu*. Two-Step adaptive laboratory evolution enhances osmotolerance in engineered Escherichia coli for improved succinate biosynthesis. Biotechnology Journal. 2025. 20(4):e70021. (JCR Q2)
7. Keming Song#, Jianing Li#, Xiaoyan Zhang, Liliang Chu, Peiwen Liu, Zhiwen Lu, Hui Wu*, Yunpeng Bai*. Enzyme-catalyzed efficient synthesis of enantiomerically pure (S)- and (R)-n-butylphthalide. ChemBioChem. 2025. 26:e202500482. (JCR Q3)
8. Binghao Zhang#, Pengye Guo#, Xinye Sun, Yanzhe Shang, Yuanchan Luo, Hui Wu*. Enhancement of lactate fraction in poly(lactate-co-3-hydroxybutyrate) biosynthesized by metabolically engineered E. coli. Bioresources and Bioprocessing. 2024. 11:88 (JCR Q1)
9. Xinye Sun#, Yanzhe Shang#, Binghao Zhang, Pengye Guo, Yuanchan Luo, Hui Wu*. Engineering of fast-growing Vibrio natriegens for biosynthesis of poly(3-hydroxybutyrate-co-lactate). Bioresources and Bioprocessing. 2024. 11:82. (JCR Q1)
10. Jiaping Jiang#, Yuanchan Luo#, Peng Fei, Zhengtong Zhu, Jing Peng, Juefeng Lu, Du Zhu, Hui Wu*. Effect of adaptive laboratory evolution of engineered Escherichia coli in acetate on the biosynthesis of succinic acid from glucose in two-stage cultivation. Bioresources and Bioprocessing. 2024. 11:34. (JCR Q1)
11. Ju Wu#, Xuan Gong#, Xinye Sun, Pengye Guo, Yuanchan Luo, Hui Wu*. Fine-tuning biosynthesis of biodegradable poly(3-hydroxybutyrate-co-lactate) with different ratios of D-lactate from xylose by engineered Escherichia coli. ACS Sustainable Chemistry & Engineering. 2023. 11:15043–15051. ( JCR Q1)
12. Mingcheng Xu#, Yuanchan Luo#, Xiaozhen Liu#, Peng Fei, Juefeng Lu, Yuying Wang, Hui Wu*. Production of 3-hydroxypropionic acid from syngas-derived acetic acid by engineered Vibrio natriegens combined with adaptive laboratory evolution. ACS Sustainable Chemistry & Engineering. 2023. 11:4125–4132. (JCR Q1)
13. Yuying Wang#, Juefeng Lu#, Mingcheng Xu, Chen Qian, Fei Zhao, Yuanchan Luo*, Hui Wu*. Efficient biosynthesis of isopropanol from ethanol by metabolically engineered Escherichia coli. ACS Sustainable Chemistry & Engineering. 2022.10: 13857-13864. (JCR Q1)
14. Juefeng Lu, Yuying Wang, Mingcheng Xu, Yuanchan Luo, Hui Wu*. Efficient biosynthesis of 3-hydroxypropionic acid from ethanol in metabolically engineered Escherichia coli. Bioresource Technology. 2022, 363:127907. (JCR Q1)
15. Yuanchan Luo, Lei Chen, Zhibo Lu, Weijian Zhang, Wentong Liu, Yuwei Chen, Xinran Wang, Wei Du, Jinyan Luo*, Hui Wu*. Genome sequencing of biocontrol strain Bacillus amyloliquefaciens Bam1 and further analysis of its heavy metal resistance mechanism. Bioresources and Bioprocessing. 2022, 9:74. (JCR Q1)
16. Dongqing Zhou, Yeling Luo, Yingqiu Zhang, Chenni Zhao, Hui Wu*, Jianhua Fan*. Perturbing electron carriers of green algae Chlamydomonas reinhardtii for efficient physiological and metabolic regulation. Food Bioengineering. 2022, 1: 148-158. (ESCI)
17. Ju Wu#, Xiangju Wei#, Pengye Guo, Aiyong He, Jiaxing Xu, Mingjie Jin, Yanjun Zhang, Hui Wu*. Efficient P(3HB-co-LA) production from corn stover hydrolysate by metabolically engineered Escherichia coli. Bioresource Technology. 2021, 341:125873. (JCR Q1)
18. Peng Fei#, Yuanchan Luo#, Ningyu Lai, Hui Wu*. Biosynthesis of (R)-3-hydroxybutyric acid from syngas-derived acetate in engineered Escherichia coli. Bioresource Technology. 2021, 336:125323. (JCR Q1)
19. Ningyu Lai#, Yuanchan Luo#, Peng Fei, Peng Hu, Hui Wu*. One stone two birds: biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetate in Escherichia coli. Synthetic and Systems Biotechnology. 2021, 6: 144-152. (JCR Q1)
20. Jianhua Fan, Dongqing Zhou, Cheng Chen, Ju Wu, Hui Wu*. Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis. Synthetic and Systems Biotechnology. 2021, 6: 351-359. (JCR Q1)
21. Pengye Guo#, Yuanchan Luo#, Ju Wu, Hui Wu*. Recent advances in the microbial synthesis of lactate-based copolymer. Bioresources and Bioprocessing. 2021, 8: 106. (JCR Q1)
22. Na Zhang♯, Hui Wu♯, Yingzhi Liang♯, Jianming Ye, Huan Zhang, Yuqing Miao, Yane Luo*, Haiming Fan , Tianli Yue. Design and preparation of “corn-like” SPIONs@DFK-SBP-M13 assembly for improvement of effective internalization.  International Journal of Nanomedicine. 2021. 16:7091-7102. (JCR Q1).
23. Xiangju Wei, Ju Wu, Pengye Guo, Shengmin Zhou, Hui Wu*. Effect of short-chain thioesterase deficiency on p(3HB-co-LA) biosynthesis in Escherichia coli. Chinese Journal of Biotechnology. 2021. 37(1):196-206.
24. Guohui Li, Dixuan Huang, Xue Sui, Shiyun Li, Bing Huang, Xiaojuan Zhang*, Hui Wu*, Yu Deng*. Advances in microbial production of medium-chain dicarboxylic acids for nylon materials. Reaction Chemistry & Engineering. 2020. 5:221-238 (JCR Q2)
25. Hao Yang, Can Zhang, Ningyu Lai, Bing Huang, Peng Fei, Dawei Ding, Peng Hu, Yang Gu, Hui Wu*. Efficient isopropanol biosynthesis by engineered Escherichia coli using biologically produced acetate from syngas fermentation. Bioresource Technology. 2020. 296:122337. (JCR Q1)
26. Qiaofei He, George N. Bennett, Ka-Yiu San, Hui Wu*. Biosynthesis of medium-chain ω-hydroxy fatty acids by AlkBGT of Pseudomonas putida GPo1 with native FadL in engineered Escherichia coli. Frontiers in Bioengineering and Biotechnology. 2019. 7:273. (JCR Q1)
27. Hao Yang#, Bing Huang#, Ningyu Lai, Yang Gu, Zhimin Li, Qin Ye, Hui Wu*. Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate. Microbial Cell Factories. 2019. 18:6. (JCR Q1)
28. Jingxian Lu, Zhimin Li, Qin Ye, Hui Wu*. Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli. Chinese Journal of Biotechnology. 2019. 35(1):59-69.
29. Jiapeng Tang, Zhenqing Qian, Hui Wu*. Enhancing cordycepin production in liquid static cultivation of Cordyceps militaris by adding vegetable oils as the secondary carbon source. Bioresource Technology. 2018. 268:60-67. (JCR Q1)
30. Bing Huang, Hao Yang, Guochen Fang, Xing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering. 2018. 115:943-954. (*Co-corresponding) (JCR Q2)
31. Wei Li#, Hui Wu#, Mai Li, Ka-Yiu San*. Effect of NADPH availability on free fatty acid production in E. coli. Biotechnology and Bioengineering. 2018. 115:444-452. (JCR Q2)
32. Han Liu, Guochen Fang, Hui Wu*, Zhimin Li*, Qin Ye. L-cysteine production in Escherichia coli based on rational metabolic engineering and modular strategy. Biotechnology Journal. 2018. 13:e1700695. (*Co-corresponding) (JCR Q1)
33. Qing Li#, Bing Huang#, Qiaofei He, Jingxian Lu, Xun Li, Zhimin Li, Hui Wu*, Qin Ye. Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation. Bioresources and Bioprocessing. 2018. 5:41. (Feature Article) (JCR Q1)
34. Jiaqi Jiang, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresources and Bioprocessing. 2018. 5:34. (*Co-corresponding) (JCR Q1)
35. Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 2017. 231:75-84. (JCR Q1)
36. Xing Zhang#, Hui Wu#, Bing Huang, Zhimin Li*, Qin Ye. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 2017. 241:163-169. (# First two authors contributed to this paper equally) (JCR Q2)
37. Qing Li#, Hui Wu#, Zhimin Li, Qin Ye*. Enhanced succinate production from glycerol by engineered Escherichia coli strains. Bioresource Technology. 2016. 218:217-223. (# First two authors contributed to this paper equally) (JCR Q1)
38. Yunjie Li, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. Production of succinate from acetate by metabolically engineered Escherichia coli. ACS Synthetic Biology. 2016. 5:1299-1307. (*Co-corresponding) (JCR Q1)
39. Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 2016. 100(14):6279-6289. (*Co-corresponding) (JCR Q1)
40. Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 2016. 15:38. (*Co-corresponding) (JCR Q1)
41. Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 2016. 43:45-53. (*Co-corresponding) (JCR Q2)
42. Cheng Wang, Jing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 2015. 214:63-68. (*Co-corresponding). (JCR Q2)
43. Hui Wu, Leepika Tuli, George N. Bennett, Ka-Yiu San*. Metabolic transistor strategy for controlling electron transfer chain in Escherichia coli. Metabolic Engineering. 2015. 28:159-168. (JCR Q1)
44. Hui Wu, George N. Bennett, Ka-Yiu San*. Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation. Biotechnology and Bioengineering. 2015. 112(8):1720-1726. (JCR Q2)
45. Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Simultaneous utilization of glucose and mannose from woody hydrolysate for free fatty acid production by metabolically engineered Escherichia coli. Bioresource Technology. 2015. 185:431-435. (JCR Q1)
46. Hui Wu, Mukund Karanjikar, Ka-Yiu San*. Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol. Metabolic Engineering. 2014. 25:82-91. (JCR Q1)
47. Hui Wu, Ka-Yiu San*. Efficient odd numbered straight-chain free fatty acid production by metabolically engineered Escherichia coli. Biotechnology and Bioengineering. 2014. 111(11):2209-2219. (JCR Q2)
48. Yane Luo*, Tao Zhang, Hui Wu*. The transport and mediation mechanisms of the common sugars in Escherichia coli. Biotechnology Advances. 2014. 32:905-919. (JCR Q1)
49. Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Efficient free fatty acid production from woody biomass hydrolysate in metabolically engineered Escherichia coli. Bioresource Technology. 2014. 169:119-125. (JCR Q1)
50. Hui Wu, Ka-Yiu San*. Engineering Escherichia coli for odd straight medium chain free fatty acid production. Applied Microbiology and Biotechnology. 2014. 98(19):8145-8154. (JCR Q1)
51. Hui Wu, Qing Li, Zhi-min Li*, Qin Ye*. Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator. Bioresource Technology. 2012. 107:376-384. (JCR Q1)
52. Yuan Liu♯, Hui Wu♯, Qin Li, Xuwei Tang, Zhi-min Li*, Qin Ye. Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source. Enzyme and Microbial Technology. 2011. 49(5):459-64. (♯ First two authors contributed to this paper equally) (JCR Q2)
53. Hui Wu, Zhi-min Li, Li Zhou, Jingli Xie, Qin Ye*. Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenetic carbon sources. Enzyme and Microbial Technology. 2009. 44:165-169. (JCR Q2)
54. Hui Wu, Zhi-min Li, Li Zhou, Qin Ye*. Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture. Applied and Environmental Microbiology. 2007. 73:7837-7843. (JCR Q1)

1．Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang. KAS III-free fatty acid synthesis. US Patent Number: 10851393B2. 授权.
2．Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 10,011,839 B2. 授权.
3．Ka-Yiu San, Xixian Xie, Leepika Tuli, Hui Wu. Hydroxy- and dicarboxylic-fat synthesis by microbes. US patent Number: 9,487,804 B2. 授权.
4．Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 9,441,253 B2. 授权.
5. 罗远婵，吴辉. 一种高耐镉的生防解淀粉芽孢杆菌及其应用. 中国发明专利. 专利申请号：202110666584. 授权.
6. 范建华，吴辉，周冬青. 一种转基因微藻及其在调控代谢产物合成中的应用. 中国发明专利，专利号：ZL202110303222.0. 授权.
7．吴辉，陆静娴，李志敏，叶勤. 一种提高大肠杆菌合成的聚（3-羟基丁酸-co-乳酸）中乳酸组分含量的方法.中国发明专利. 专利号：ZL201810246568.X. 授权.
8．吴辉，杨昊，李志敏，黄兵，叶勤. 利用乙酸生产丙酮或异丙醇的代谢工程大肠杆菌菌株的构建方法与应用. 中国发明专利. 专利号：ZL201710978174.9. 授权.
9．吴辉，黄兵，李志敏，杨昊，叶勤. 利用乙酸生产羟基丙酸的代谢工程大肠杆菌菌株的构建方法与应用. 中国发明专利. 专利号：ZL201710969403.0. 授权.
10．叶勤，李晴，李志敏，吴辉. 利用重组大肠杆菌以甘油为碳源发酵生产琥珀酸的方法. 中国发明专利. 专利号：ZL201610305322.6. 授权.
11．李志敏, 黄兵, 吴辉, 房国辰, 叶勤.一种利用造纸废液生物合成丁二酸的方法. 中国发明专利. 专利号: ZL201711142577.6. 授权
12．李志敏, 李运杰, 吴辉, 叶勤. 利用乙酸生产丁二酸的代谢工程大肠杆菌菌株构建方法和应用. 中国发明专利. 专利号：ZL201410599042.1. 授权.
13．孙周通，刘映淼，孙兵兵，王祎，吴辉，杨俊杰等. L-色氨酸基因工程菌,其构建方法以及使用其发酵生产L-色氨酸的方法. 中国发明专利. 专利号：ZL201010598350. 授权.
14．叶勤，李志敏，吴辉. 利用四碳二元酸发酵固定二氧化碳的方法. 中国发明专利. 专利号：ZL200810207564. 授权.
15．吴辉，李志敏，叶勤. 大肠杆菌生产琥珀酸的方法. 中国发明专利. 专利号：ZL200710135684. 授权.

1．主持国家重点研发计划子课题2026.01 - 2029.12
2．主持国家自然科学基金面上基金2023.01 - 2026.12
3．主持国家重点研发计划课题2022.11 - 2026.10
4．主持国家重点研发计划子课题2021.12 - 2024.11
5．主持国家自然科学基金面上基金2018.01 - 2021.12
6．主持国家重点研发计划子课题2017.07 - 2021.06
7．主持霍英东教育基金会第十六届高等院校青年教师基金2018.3-2021.3
8．主持上海市自然科学基金2019.6-2022.6
9．主持国家自然科学基金青年科学基金2015.1-2017.12

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