精细化工,生物医药

1) 生物催化剂的挖掘与改造
2) 工业生物过程技术集成与产业化

1. Yin DY#, Pan J (共同一作)#, Zhu J,  Liu YY, Xu JH*. A green-by-design bioprocess for L-carnosine production integrating enzymatic synthesis with membrane separation. Catal. Sci. Technol., 2019, 9: 5971–5978.
2. Zhang Y#, Pan J(共同一作)#, Luan ZJ, Xu GC*, Xu JH*, Park SH. Promiscuous esterase activity of a novel bacterial chloroperoxidase for highly enantioselective synthesis of a chiral cilastatin precursor. Appl. Environ. Microbiol., 2014, 80:7348-7355.
3. Liu CH#, Pan J(共同一作) #, Ye Q, Xu JH*. Enzymatic production of Cilastatin intermediate via highly enantioselective hydrolysis of methyl (±)-2,2-dimethylcyclopropane carboxylate using newly isolated Rhodococcus sp. ECU1013. Appl. Microbiol. Biotechnol., 2013, 97: 7659–7667.
4. Chen C, Xuan Y, Chen Q, Ni GW, Pan J*, Xu JH*. Asymmetric reduction of 2-chloro-3-oxo-ester into enantiomerically high pure diltiazem precursor by a Candida ketoreductase. Mol. Catal., 2021, 510: 111670.
5. Zhang LJ, Hu P, Pan J*, Yu HL*, Xu JH. Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation.Chin. J. Chem. Eng. 2021, 29: 311-316.
6. Sun QF, Zheng YC, Chen Q, Xu JH*, Pan J*. Engineering of an oleate hydratase for efficient C10-Functionalization of oleic acid. Biochem. Biophys. Res. Commun., 2021, 537: 64-70.
7.  Wu YX, Pan J*, Yu HL, Xu JH*. Enzymatic synthesis of 10-oxostearic acid in high space-time yield via cascade reaction of a new oleate hydratase and an alcohol dehydrogenase. J. Biotechnol.: X, 2019, 2：N100008.
8. Jiao XC, Pan J*, Kong XD, Xu JH*. Protein engineering of aldolase LbDERA for enhanced activity toward real substrates with a high-throughput screening method coupled with an aldehyde dehydrogenase. Biochem. Biophys. Res. Commun., 2017, 482: 159−163.
9.  Jiao XC, Zhang YJ, Chen Q, Pan J*, Xu JH*. A green-by-design system for efficient bio-oxidation of an unnatural hexapyranose into chiral lactone for building statin side-chains. Catal. Sci. Technol., 2016, 6(19): 7094-7100.
10. Jiao XC, Pan J*, Xu GC, Kong XD, Chen Q, Zhang ZJ, Xu JH*. Efficient synthesis of statin precursor in high space-time yield by a new aldehyde-tolerant aldolase identified from Lactobacillus brevis.Catal. Sci. Technol., 2015, 5: 4048-4054
11. Chen J, Luo XJ, Chen Q, Pan J*, Zhou J, Xu JH*. Marked enhancement of Acinetobacter sp. organophosphorus hydrolase activity by a single residue substitution Ile211Ala. Bioresour. Bioprocessing, 2015, 2: 39.
12. Zhou DJ, Pan J*, Yu HL, Zheng GW, Xu JH*. Target-oriented discovery of a new esterase-producing strain Enterobacter sp. ECU1107 for whole cell-catalyzed production of (2S,3R)-3-phenylglycidate as a chiral synthon of Taxol. Appl. Microbiol. Biotechnol., 2013, 97(14): 6293-6300.
13. Ju X, Yu HL, Pan J*,Xu JH*. Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation. Bioprocess Biosys. Eng., 2012, 35: 323–331.

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国家重点研发项目：生物基聚氨酯绿色制造技术的开发与产业化示范 (2021YFC2101900)子课题
国家自然科学基金项目：γ-/δ-长链酮酸还原酶的创制及在手性内酯香料合成中的应用(21778065)
科技部“863”计划项目：生物催化还原制备手性芳基醇关键技术研究(2006AA02Z205) 生物催化合成芳香手性醇基内酰胺(2007AA02Z225)
企业合作项目：L-肌肽酶法合成产业化技术研发；手性合成用脂肪酶制剂的开发；10-羟基硬脂酸酶法合成研发；酶法生产(2S)-顺-羟基内酰胺中试技术；他汀药物手性中间体的酶法合成

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