生物医药

（1） 工业微生物发酵生理与分子调控：主要对工业微生物在发酵过程中与培养环境之间的相互作用关系，包括环境条件对微生物细胞在发酵过程的生理变化影响机制，如碳中心代谢途径的改变引起代谢产物的合成效率；同时，研究细胞发酵生理对培养环境的影响，为建立工业化发酵过程控制提供理论基础。
（2） 动物细胞大规模培养工程：主要对CHO细胞、BHK21、HEK293、Vero细胞、干细胞（如骨髓间充质干细胞、小鼠胚胎干细胞、神经干细胞等）、原代组织细胞（如睾丸支持细胞等）等生物反应器大规模培养与抗体（疫苗）工程化技术研究，建立高密度的生物反应器培养技术和在线检测技术，以及大规模生物反应器设计技术研究。

1. 应用与环境微生物分子生理与过程工程方向
（1） Hong-Tu Zhao, Kun-Yun Pang, Wei-Lu Lin, Ze-Jian Wang, Da-Qi Gao, Mei-Jin Guo*,Ying-Ping Zhuang. Optimization of the n-propanol concentration and feedback controlstrategy with electronic nose in erythromycin fermentation processes. Process Biochemistry,2015,  dx.doi.org/10.1016/j.procbio.2015.12.001
（2） Zhi-Qiang Xiong, Mei-Jin Guo*, Ju Chu, Ying-Ping Zhuang, Si-liang Zhang, On-line specific growth rate control for improving reduced glutathione production in Saccharomyces cerevisiae. Biotechnology & Bioprocess Engineering. 2015,20(5):887-893
（3） Taicheng Zhu, Haifeng Hang, Ju Chu, Yingping Zhuang, Siliang Zhang and Meijin Guo*. Transcriptional investigation of the effect of mixed feeding to identify the main cellular stresses on recombinant Pichia pastoris. Journal of Industrial Microbiology & Biotechnology. 2013: 40:183-189
（4） Lan Yu, Xiangyun Yan, Long Wang, Ju Chu, Yingping Zhuang, Siliang Zhang and Meijin Guo*. Molecular cloning and functional characterization of an ATP-binding cassette transporter OtrC from Streptomyces rimosus. BMC Biotechnology 2012, 12:52
（5） Xiaohe Chu, Zijing Zhen, Zhenyu Tang, Yingping Zhuang, Ju Chu, Siliang Zhang1 and Meijin Guo*. Introduction of extra copy of oxytetracycline resistance gene otrB enhances the biosynthesis of oxytetracycline in Streptomyces rimosus. J Bioprocess Biotechniq 2012, 2:117
（6） Lan Yu, Nan Cao, Long Wang, Ciying Xiao, Meijin Guo*, Ju Chu, Yingping Zhuang, Siliang Zhang. Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes, Enzyme and Microbial Technology,  2012,50(6):318–324
（7） Zhenyu Tang, Paul R. Herron, Iain S. Hunter, Siliang Zhang and Meijin Guo*. Insight into microevolution of Streptomyces rimosus based on analysis of zwf and rex genes. African Journal of Microbiology Research ,2012,6(24):5229-5236
（8） Zhenyu Tang, Ciying Xiao, Yingping Zhuang, Ju Chu, Siliang Zhang, Paul R. Herron, Iain S. Hunter, Meijin Guo*.Improved oxytetracycline production in Streptomyces rimosus M4018 by metabolic engineering of the G6PDH gene in the pentose phosphate pathway. Enzyme and Microbial Technology, 2011,49( 1): 17–24
（9） Zhenyu Tang, Yingping Zhuang, Ju Chu, Siliang Zhang, Paul Herron, Iain S. Hunter and Meijin Guo*.  A molecular Redox Sensor from Streptomyces rimosus M4018 for Escherichia coli . African Journal of Microbiology Research, 2011, 5(31): 5682-5688
（10） Florence E. Pethick, Alison C. MacFadyen, Zhenyu Tang, Vartul Sangal, Tze-Tze Liu, Ju Chu, Gregor Kosec, Hrvoje Petkovic, Meijin Guo, Ralph Kirby, Paul A. Hoskisson, Paul R. Herron, Iain S. Hunter. Draft genome sequence of the oxytetracycline-producing bacterium Streptomyces rimosus ATCC 10970. Genome Announc. 2013,1(2): e00063-13. doi: 10.1128/genomeA.00063-13. PMCID: PMC3593331
（11） Taicheng Zhu , Meijin Guo* , Yingping Zhuang ,Ju Chu and Siliang Zhang. Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes. Appl Microbiol Biotechnol. 2011, 89:1127–1135
（12） Zhi-Qiang Xiong, Mei-Jin Guo*, Yuan-Xin Guo, Ju Chu, Ying-Ping Zhuang, Nam Sun Wang and Si-Liang Zhang. RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65. Enzyme and Microbial Technology. 2010, 46 (7): 598-602
（13） Taicheng Zhu, Meijin Guo*, Ming Zhang, Zhenyu Tang, Yingping Zhuang, Ju Chu, Siliang Zhang. Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeast Pichia pastoris, Journal of Applied Microbiology . 2009,107: 954–963
（14） Taicheng Zhu, Meijin Guo*, Chen Sun, JuChu, Yingping Zhuang, SiliangZhang.A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains.Biotechnol Lett. 2009. 31:679-684
（15） Haifeng Hang, Xinhao Ye, Meijin Guo*, Ju Chu, Yingping Zhuang, Ming Zhang, Siliang Zhang. A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate. Enzyme and Microbial Technology 2009,44:185–188
（16） Zhiqiang Xiong, Meijin Guo*, Yuanxin Guo, Ju Chu, Yingping Zhuang, Siliang Zhang, Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol, Bioresource Technology, 2009, 100 :1011–1014
（17） Zhiqiang Xiong, Meijin Guo*, Yuanxin Guo, Ju Chu,Yingping Zhuang and Siliang Zhang. Real time viable cell mass monitoring in high-cell-density fed-batch glutathione fermentation with industrial complex medium by Saccharomyces cerevisiae T65. Journal of Bioscience and Bioengineering, 2008, 105:409-413
（18） Xiao-Qing Hu, Ju Chu, Zhuo Zhang, Si-Liang Zhang, Ying-Ping Zhuang, Yong-Hong Wang, Meijin Guo*, Hua-Xin Chen, Zhong-Yi Yuan. Effects of different glycerol feeding strategies on S-adenosyl-L-methionine biosynthesis by PGAP-driven Pichia pastoris overexpressing methionine adenosyltransferase. Journal of Biotechnology ,2008, 137(1-4):44-49
（19） Haifeng Hang, Wen Chen, Meijin Guo*, Ju Chu, Yingping Zhuang, Siliang Zhang, A simple unstructured model-based control for efficient expression oe recombination porcine insulin precursor by Pichia pastoris, Korean Journal of Chemical Engineering. 2008, 25(5):1065-1069
（20） Meijin Guo, Haifeng Hang, Taicheng Zhu, Yingping Zhuang*, Ju Chu, Siliang Zhang. Effect of glycosylation on biochemical characterization of recombinant phytase expressed in Pichia pastoris. Enzyme and Microbial Technology, 2008,43:334-338
（21） Meijin Guo, Yingping Zhuang*, Ju Chu, Siliang Zhang, Aisheng Xiong, Rihe Peng, Quanhong Yao. Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34. Process Biochemistry, 2007,42:1660-1665
（22） Yaming Dong, Qinqin Fan, Xuefeng Yan*, Meijin Guo, Fei Lu. Development of a hybrid model for sodium gluconate fermentation by Aspergillus niger. Journal of Chemical Technology and Biotechnology, 2014, 89(12): 1875-1882
（23） Qingchao Jiang, Xuefeng Yan*, Zhaomin Lv, Meijin Guo. Fault detection in nonlinear chemical processes based on kernel entropy component analysis and angular structure. Korean Journal of Chemical Engineering, 2013, 30(6): 1181-1186
（24） Wang, L., Xiao, L. H., Duan, L. P., Ye J. B., Guo, Y. Q., Guo, M. J., Liu, L. L., Feng, Y. Y.* (2013) Concurrent infections of Giardia duodenalis, Enterocytozoon bieneusi, and Clostridium difficile in children during a cryptosporidiosis outbreak in a pediatric hospital in China. PLoS Neglected Tropical Diseases. 7, e2437
（25） Lin Wang, Hongwei Zhang, Xudong Zhao，Longxian Zhang,Guoqing Zhang, Meijin Guo, Lili Liu, Yaoyu Feng and Lihua Xiao. Zoonotic  cryptosporidium species and Enterocytozoon bieneusi genotypes in HIV-positive patients on antiretroviral therapy. Clin. Microbiol. 2013,51: 2 557-563. doi:10.1128/JCM.02758-12J.
（26） Li, N., Xiao, L. H., Cama, V. A., Ortega, Y., Gilman, R. H., Guo, M. J., Feng, Y. Y.* (2013) Genetic recombination and Cryptosporidium hominis virulent subtype IbA10G2. Emerging Infectious Diseases. 19, 1573-1582
（27） Li, N., Xiao, L. H.*, Wang, L., Zhao, S. M., Zhao, X. K., Duan, L. P., Guo, M. J., Liu, L. L., Feng, Y. Y.* (2012) Molecular surveillance of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi by genotyping and subtyping parasites in wastewater. PLoS Neglected Tropical Diseases. 6: e1809
（28） Ye, J. B., Xiao, L. H., Ma, J. B., Guo, M. J., Liu, L. L., Feng, Y. Y.* (2012)  Anthroponotic enteric parasites in monkeys in public park, China. Emerging Infectious Diseases. 18: 1640-1643 (IF: 6.751) (Q1 in Infectious Diseases) (was selected as the cover story of the issue 10, 2012)
（29） Zhang, Wei; Sun, Wenfang; An, Shuai; Xiong, Bang; Lin, Kuangfei; Cui, Xinhong; Guo, Meijin . Acute and chronic toxic effects of chloramphenicol on Scenedesmus obliquus and Chlorella pyrenoidosa. Water Environment Research, ,2013 , 85, 8: 725-732
（30） Wei Zhang, Bang Xiong, Lin Chen, Kuangfei Lin, Xinhong Cui, Huasong Bi, Meijin Guo,Weiliang Wang.  Toxicity assessment of Chlorella vulgaris and Chlorella protothecoides following exposure to Pb(II). Environmental Toxicology and Pharmacology. 2013, 36(1):51–57
（31） Wei Zhang, Meng Zhang, Kuangfei Lin, Wenfang Sun, Bang Xiong,Meijin Guo, Xinhong Cui, Rongbing Fu. Eco-toxicological effect of Carbamazepine on Scenedesmus obliquus and Chlorella pyrenoidosa. Environmental Toxicology and Pharmacology. 2012, 33 (2): 344–352
（32） Wei Zhang, Li Li, Kuangfei Lin, Bang Xiong, Bingzhi Li, Shuguang Lu, Meijin Guo and Xinhong Cui. Synergetic degradation of Fe/Cu/C for groundwater polluted by trichloroethylene. Water Science & Technology, 2012, 65(12):2258–226, doi:10.2166/wst.2012.146
（33） Wei Zhang, Kuangfei Lin, Youna Miao, Qiaoxiang Dong, Changjiang Huang, Huili Wang, Meijin Guo, Xinhong Cui. Toxicity assessment of zebrafish following exposure to CdTe QDs. Journal of Hazardous Materials, 2012,30:413–420

2. 动物细胞培养与生物反应器设计
（1） Huan Tian, Meijin Guo*, Yingping Zhuang, Ju Chu, Siliang Zhang. Enhanced proliferation of bone marrow mesenchymal stem cells by co-culture with TM4 mouse Sertoli cells: involvement of the EGF/PI3K/AKT pathway. Molecular and cellular biochemistry, 2014, 393(1-2): 155-164
（2） Lei Deng, Bingyang Shi, Yingping Zhuang, Ju Chu, Xiaolin Shi, Siliang Zhang, and Meijin Guo*. Performance and mechanism of neuroleukin in the growth and survival of Sertoli cell induced-neurons in the co-culture system. Cell Transplantation, 2013
（3） Lang Zhou, Jiantao Kong, Yingping Zhuang, Ju Chu, Siliang Zhang, and Meijin Guo*. Ex vivo expansion of bone marrow mesenchymal stem cells using microcarrier beads in a stirred bioreactor. Biotechnology and Bioprocess Engineering, 18: 000-000, 2013
（4） Bingyang Shi, Lei Deng, Xiaolin Shi, Sheng Dai, Hu Zhang, Yonghong Wang, Jingxiu Bi, Meijin Guo* The enhancement of neural stem cell survival and growth by coculturing with expanded sertoli cells in vitro. Biotechnology Progress, 2012, 28(1): 196-205
（5） Bingyang Shi, Lei Deng, Xiaolin Shi, Sheng Dai, Hu Zhang, Yonghong Wang, Jingxiu Bi, Meijin Guo*. The enhancement of neural stem cell survival and growth by coculturing with expanded sertoli cells in vitro. Biotechnology Progress, 2012, 28(1): 196- 205
（6） Haifeng Hang, Yuanxin Guo, Jian Liu, Li Bai, Jianye Xia, Meijin Guo* and Matthew Hui.Computational fluid dynamics modeling of an inverted frustoconical shaking bioreactor for mammalian cell suspension culture. Biotechnology and Bioprocess Engineering, 2011, 16(3): 567-575
（7） Bingyang Shi, Suxiang Zhang, Meijin Guo*, Yonghong Wang,Yingping Zhuang, Ju Chu, Siliang Zhang and Xiaolin Shi. Expansion of Mouse Sertoli Cells on Microcarriers. Cell Proliferation. 2010,43,275–286
（8） Suwen Hu, Lei Deng, Huamao Wang, Yingping Zhuang*, Ju Chu, Siliang Zhang, Zhonghai Li and Meijin Guo*. Bioprocess development for the production of mouse-human chimeric anti-epidermal growth factor receptor vIII antibody C12 by suspension culture of recombinant Chinese hamster ovary cells. Cytotechnology, 2011, 63(3), 247-258
（9） Haifeng Hang, Yuanxin Guo, Jian Liu, Li Bai, Jianye Xia, Meijin Guo* and Matthew Hui.Computational fluid dynamics modeling of an inverted frustoconical shaking bioreactor for mammalian cell suspension culture.Biotechnology and Bioprocess Engineering, 2011, 16(3): 567-575

（1） 郭美锦，张嗣良，于岚，颜湘云，王龙，储炬，庄英萍，一种提高土霉素产量的方法，授权日:2015.6.17，中国，ZL201210071770.6.
（2） 郭美锦;郑子静;姚高峰;储炬;庄英萍;张嗣良;钱江潮. 一种增加龟裂链霉菌的土霉素产量的方法, 申请日期：2011年4月27日, 专利申请号： 201110107321.8, 授权日：2015年3月4日
（3） 郭美锦，张嗣良，储炬，杭海峰， 一全合成培养基及其应用方法，授权日:2001，10.09，中国，ZL 01 1 05699.1
（4） 储炬，李啸，张嗣良，庄英萍，钟承赞，葛友群，杭海峰，王永红，郭美锦，夏建业，章付林，杨明. 利用铵离子调控林可霉素发酵过程及提高林可霉素产素，申请日期2008年1月23日，申请号：200810032954.5，公开号：CN101220385.A，专利号：ZL200810032954.5
（5） 张嗣良, 王公先, 郭美锦, 唐寅, 马文峰, 张明, 张淑香, 刘健, 顾寅, 张剑坤: 无泡通气系统在位消毒装置. CN 201020521866.4
（6） 张嗣良, 王公先, 郭美锦, 唐寅, 马文峰, 张明, 张淑香, 刘健, 顾寅, 张剑坤: 一种带有无泡通气系统的动物细胞反应器. 申请号CN 201010275887.7
（7） 张嗣良, 张岳芳, 刘健, 王绍娟, 郭美锦, 贺莉清, 张恂, 张明, 袁旭军, 马文峰: 在线细胞显微观察仪. 申请号CN 200720071207.3
（8）  张嗣良, 张岳芳, 刘健, 王绍娟, 郭美锦, 贺莉清, 张恂, 张明, 袁旭军, 马文峰: 用于生化反应器的在线细胞显微观察仪. 申请号CN 200710042189.0

1. 国家“863”计划项目，2012AA021201，生物制造反应过程技术与装备，2012/01-2015/12，经费：1605万（国拨905万），主持。
2. 国家973计划《能源微藻规模化生产的科学基础》子课题 “多参数全自动封闭式光生物反应器设计与优化原理” 编号：2011CB200903，2011.01—2015.8，经费：50万，负责人。
3. 国家973计划《能源微藻规模化生产的科学基础》子课题 “微藻光自养动力学、多参数分析与过程优化” ，编号：2011CB200904，2011.01—2015.8，经费：30万，负责人。
4. 国家“863”计划项目，2008AA02Z201，符合cGMP标准的哺乳动物细胞生物反应器的研制，2008/01-2010/12，经费：100万，主持。
5. 国家自然科学基金面上项目，30560006，重组毕赤酵母不同基因型与培养表型特征相关研究，2006/01-2008/12，经费：22万，已结题，主持。

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