精细化工,生物医药

1．代谢工程：微生物代谢过程分析、调控及育种。应用于基因工程蛋白药物的制备与研发以及其它生物技术药物的发酵制备过程研究。
2．生物催化与转化：微生物新功能酶源的筛选和性能研究;生物催化反应的工业化应用。

生物催化剂和生物催化过程应用关键技术，上海市科技进步三等奖（2005）
生物催化法制备单唾液酸神经节苷脂GM1，上海市科技进步奖三等奖(2006)
应用生物催化与生物分离技术发现和获得新生物活性物质的研究，上海市自然科学奖三等奖(2007)
工业酶制剂的开发与应用关键技术。上海市科技进步奖一等奖， 2010
定向转化多元醇的细胞催化剂创制及其应用关键技术。上海市技术发明奖一等奖，2013
定向转化多元醇的生物催化剂创制及其应用关键技术，国家技术发明奖二等奖，2015

1. Xu Xinwei, Gao Xingqiang, Feng Jianxun, Wang Xuedong*, Wei Dongzhi. Influence of temperature on nucleus degradation of 4-androstene-3,17-dione in phytosterol biotransformation by Mycobacterium sp. Letters in Applied Microbiology. 2015, 61: 63-68.
2. Wang Hualei, Gao Wenyuan, Sun Huihui, Chen Lifeng, Zhang Lujia, Wang Xuedong*, Wei Dongzhi*. Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid. Applied and Environmental Microbiology. 2015, 81(24)：8469-8477.
3.  Gao Xingqiang, Feng Jianxun, Hua Qiang, Wang Xuedong*, Wei Dongzhi. Enhanced steroid metabolites production by resting cell phytosterol bioconversion. Chem. Biochem. Eng. Q., 2015, 29 (4): 567-573.
4. Wang Xuedong*, Long Hui, Shen Danhong, Liu Long. Cloning, expression, and characterization of a novel sialidase from Brevibacterium casei. Biotechnology and Applied Biochemistry. 2016, 64(2): 195-200.
5.  Song Hao, Jiang Jingxin, Wang Xuedong*, Zhang Jianguo (2017) High purity recombinant human growth hormone rhGH expression in Escherichia coli under phoA promoter. Bioengineered. 2017, 8(2): 147-153
6. He Wei, Shu Jiayi, Zhang Jian, Liu Zhihua, Xu Jianqing, Jin Xia*, Wang Xuedong*. (2017) Expression, purification and renaturation of recombinant peptide-based HIV vaccine in Escherichia coli. Canadian Journal of Microbiology, 63(6): 493-501.
7. Wang Xuedong*, Hua Chaolong, Xu Xinwei, Wei Dongzhi. Two-Step Bioprocess for Reducing Nucleus Degradation in Phytosterol Bioconversion by Mycobacterium neoaurum NwIB-R10hsd4A. Applied Biochemistry and Biotechnology. 2019, 188: 138-146.
8. Wang Zhong, Zhang Jian*, Wang Xuedong*, Wei Dongzhi. High level expression and characterization of the recombinant immunotoxin DAB389-4D5 scFv targeting HER2/neu-positive ovarian carcinoma cells. Process Biochemistry. 2019, 80: 26-34.
9.  Song Yansong, Wang Xuedong*, Zhang Jianguo. Enhancement of Bioconversion Ganglioside s to Monosialotetrahexosylganglioside by In Situ Sialic Acid Removal and Recovery. Can. J. Chem. Eng. 2019, 97: 2617-2620.
10.  Wang Xuedong*, Chen Rui, Wu Yuyang, Wang Da, Wei Dongzhi. Nitrate Metabolism Decreases the Steroidal Alcohol Byproduct Compared with Ammonium in Biotransformation of Phytosterol to Androstenedione by Mycobacterium neoaurum. Applied Biochemistry and Biotechnology. 2020, 190: 1553–1560.
11.  Hu Yu,Wang Da. Wang Xuedong*. Wei Dongzhi. A recycled batch biotransformation strategy for 22-hydroxy-23,24-bisnorchol-4-ene-3-one production from high concentration of phytosterols by mycobacterial resting cells. Biotechnol Lett. 2020, 42:2589-2594.
12.   尉文，张梦雪，王学东，无机氮源在枯草芽孢杆菌补料分批发酵核黄素中的作用. 2020，46(4): 553-558.

专利名称：单唾液酸四己糖神经节苷脂的制备方法申请号：200410018280.5
专利名称：一种尼克酸生物学制备方法、相关的菌株及其腈水解酶诱导方法，申请号：200910050127.3
专利名称：一种毕赤酵母基因工程菌发酵生产S-腺苷甲硫氨酸的方法，申请号：200910052700.4
专利名称：一种生物转化植物甾醇制备甾体药物中间体的方法，申请号：201410027059.X

国家863“重大新药创制”科技重大专项：生物技术药物中试放大及分离纯化技术平台，2012ZX09304009；
国家863重大项目子课题：柠檬酸，葡萄糖酸的生物转化技术( 2011AA02A213)；
国家自然科学基金：分支杆菌植物甾醇侧链降解与辅因子循环相关性研究(面上项目，2013, No. 21276083)；微生物甾醇侧链降解过程中母核降解机制研究与改进(面上项目，2016, No. 31570079)
企业合作项目：基因工程菌发酵法制备腺苷甲硫氨酸；生物转化法制备神经节苷脂GM1；基因工程蛋白药物工艺开发；甾醇生物转化技术制备甾体药物中间体；

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