精细化工,生物医药

1. 智能化微生物制造体系构建：利用多组学解析、AI 辅助代谢路径设计与可编程合成生物学体系，实现细胞代谢网络的精准重塑，构建可按需调控与快速迭代的智能微生物工厂，实现结构复杂、高附加值天然产物的高效绿色制造。
2. 面向绿色制造的先进生物催化体系：结合计算酶学、深度学习与高通量定向进化，构建覆盖新酶挖掘—结构优化—性能提升的全链条 AI 设计体系，创制具有高活性、高稳定性和绿色工艺适配性的先进生物催化剂，支撑活性分子的可持续制造。

1. Yichen Yang#, Ming Jiang#, Shuting Hou, Shiyuan Wang, Yang Xiao, Lujia Zhang. Bei Gao*. Enzyme assembly guided via modified signal peptide for enhanced cascade biocatalysis. Journal of Agricultural and Food Chemistry. 2025, 73, 29779−29789 （封面文章）
2. Xin Yan, Ruyi Chen, Zhaoting Yuan, Zihan Lin, Xin Yang, Jiameng Zhang, Haiming Jiang, Bei Gao*, Lujia Zhang*. AI-FRET-guided engineering of subtilisin for green aqueous-phase amide bond formation with enhanced catalytic activity. Chemical Engineering Journal. 2025, 525: 169856
3. Hui Li, Yichen Yang, Shuting Hou, Lujia Zhang, Jiaying Yang, Bei Gao*. Deep learning-driven enzyme engineering in Pichia pastoris: a sustainable platform for high-yield nootkatone biosynthesis. BioFactors. 2025, 51: e70048
4. Yinghui Feng, Xin Yan, Mingzhe Ma, Ruyi Chen, Chuanxi Zhang, Yalong Cong, Bohuan Fang, Chunchi Chen, Longhai Dai, Hao Li, Haiming Jiang, Hong Sun, Hao Wei, Reyting Guo, Bei Gao*, John Z. H. Zhang*, and Lujia Zhang*. Aqualigase: a star enzyme for one-step peptide bond dehydration condensation in nature aqueous phase. ACS Catalysis. DOI:10.1021/acscatal.5c01532.
5. Wei Zhou, Ruijing Ling, Yichen Yang, Shuting Hou, Fengqing Wang*, Bei Gao*, Dongzhi Wei. Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol. Metabolic Engineering. 2025, 90:141-153
6. Rongkai Zhang, Arshia Amjad, Lujia Zhang, Wei Zhou, Yichen Yang, Qixin He, Jingli Xie, Bei Gao*. Synthesis of myrcene using a hybrid cell-free reaction system. Biochemical Engineering Journal. 2024, 208: 109361
7. Wei Zhou, Yuanyi Li, Guosong Liu, Weichuang Qin, Dongzhi Wei, Fengqing Wang, Bei Gao*. CRISPR/Cas9based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii. Applied Microbiology and Biotechnology. 2024, 108: 197
8. Yiting Zhu, Yinghui Feng, Jiawei Wang, Zhaoting Yuan, Yulu Miao, Tingwei Miao, Bei Gao*, Lujia Zhang*. Selective esterification design of lipases for TAG synthesis based on the unique structure of curved DHA. ACS Food Science & Technology. 2024, 4: 1722-1730.
9. Zidong Jiang, Lujia Zhang, Wei Zhou, Hui Li, Yuanyi Li, Weichuang Qin, Fengqing Wang, Dongzhi Wei, Bei Gao*. The rational modification of the secretion pathway: The bidirectional grinding strategy on signal peptide and SecA in Bacillus subtilis. Biochemical Engineering Journal. 2023, 191: 108769
10. Chuanxi Zhang, Yulu Miao, Yinghui Feng, Jiawei Wang, Zhuoli Tian, Juan Dong, Bei Gao*, Lujia Zhang*. Umami polypeptide detection system targeting the human T1R1 receptor and its taste-presenting mechanism. Biomaterials. 2022, 287: 121660.
11. Min Jiang, Yongjun Liu, Guosong Liu, Wei Zhou, Yaling Shen, Bei Gao*, Fengqing Wang*, Dongzhi Wei*. Sequential resolution of (S) and (R)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn. Green Chemistry. 2022, 24, 3235 – 3242
12. Tian Li, Guosong Liu, Wei Zhou, Min Jiang, Yuhong Ren, Xinyi Tao, Min Liu, Ming Zhao, Fengqing Wang*, Bei Gao*, Dongzhi Wei. Engineering ethanol utilization to overproduce squalene in Saccharomyces cerevisiae. Journal of Agriculture and Food Chemistry. 2020, 68: 2132-2138.
13. Guo-Song Liu, Tian Li, Wei Zhou, Min Jiang, Xin-Yi Tao, Min Liu, Ming Zhao, Yu-Hong Ren, Bei Gao*, Feng-Qing Wang*, Dong-Zhi Wei*. The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction. Metabolic Engineering. 2020, 57:151-161.

1. 国家重点研发计划子课题：维生素的高效异源合成，2024-2029。
2. 国家重点研发计划子课题：珍稀药用植物活性成分的合成生物学应用研究，2023-2028。
3. 上海市农业科技创新项目：桃类水果的菌酶联合精准高值化精深加工技术及其应用，2024-2027。
4. 国家自然科学基金面上项目：基于底物选择性机制解析的脂肪酶理性设计及ω-3多不饱和脂肪酸甘油酯的定向合成，2018-2021。
5. 国家自然科学基金青年项目：基于结构生物学及分子模拟方法的脂肪酶理性设计与改造，2013-2015。
6. 国家高技术发展（863）计划：啤酒用新酶创制与低碳制造关键技术研究，2013-2017。
7. 上海市自然科学基金项目，面向高值ω-3多不饱和脂肪酸酯合成的细胞工厂创建研究，2021-2024。
8. 上海市自然科学基金项目：基于结构认知和理论计算的酶分子理性设计策略研究2016-2019。
9. 企业横向课题，香精香料的生物合成，2025-2027。
10.企业横向课题，溶菌酶生产菌种和技术开发，2020-2022。
11.企业横向课题，闪式破碎仪合作开发，2021-2022。
12.企业横向课题，热启动DNA聚合酶的开发，2019-2021。