论文著作：
1. Shuting Pan, Yuguo Dong, Xuedong Wang, Yuhong Ren, Zebo Xiu*, Jian Zhang*. Site-specific PEGylation of recombinant protein SAC-TRAIL and characterization of the effect on antitumor activity. Molecular Biology Reports. 2025, 52:305
2. Min Wei, Wenxin Chen, Yuguo Dong, Yiyang Gu, Dongzhi Wei, Jian Zhang*, Yuhong Ren*. Hypoxia-inducible factor-1α-activated protein switch based on allosteric self-splicing reduces nonspecific cytotoxicity of pharmaceutical drugs. Molecular Pharmaceutics. 2024, 21:5335-47
3. Shuyan Tang, Weiting Ji, Yunqiu Zhao, Jian Zhang*, Dongzhi Wei, Fengqing Wang*. De novo biosynthesis of betulinic acid in engineered Saccharomyces cerevisiae. Bioorganic Chemistry. 2024, 152:107737
4. Yuhan Lian, Liuliu Jin, Fengqing Wang, Jian Zhang*, Yuhong Ren*. Biosynthesis of plant-derived triterpenoid asiatic acid in Saccharomyces cerevisiae cell factories. Bioorganic Chemistry. 2024, 152:107737
5. Anxiang Chen, Yuguo Dong, Huaigu Jiang, Min Wei, Yuhong Ren, Jian Zhang*. Application of plasmid stabilization systems for heterologous protein expression in Escherichia coli. Molecular Biology Reports. 2024, 51:939
6. Anxiang Chen, Yuguo Dong, Huaigu Jiang, Shengli Yang, Jian Zhang*, Dongzhi Wei. Identification and analysis of the key genes for Escherichia coli heterologous protein expression by transcriptomic profiling. Molecular Biology Reports. 2024, 51:1074
7. Xiaomei Zhu, Xiao Wang, Jian Zhang*, Xuedong Wang*. Enhancing production and purity of 9-OH-AD from phytosterols by balancingmetabolic flux of the side-chain degradation and 9-position hydroxylation in Mycobacterium neoaurum. Biotechnology Journal. 2024, 19:e2300439
8. Min Wei, Anxiang Chen, Jian Zhang*, Yuhong Ren*. A novel oxygen-dependent degradable immunotoxin regulated by ubiquitin-proteasome system reduces non-specific cytotoxicity. Molecular Pharmaceutics. 2023, 20(1):90-100
9. Dandan Cao, Xiaomei Zhu, Jian Zhang*, Xuedong Wang*. A combined strategy to enhance phytosterols biotransformation to 22-hydroxy-23,24-bisnorchol-4-ene-3-one by Mycobacterium neoaurum. Process Biochemistry. 2023, 128:94-7
10. Yuting Li, Ming Zhao, Dongzhi Wei, Jian Zhang*, Yuhong Ren*. Photocontrol of itaconic acid synthesis in Escherichia coli. ACS Synthetic Biology. 2022, 11(6):2080-8
11. Yingjie Han, Xiaomei Zhu, Xuedong Wang*, Jian Zhang*. Oxygen bioavailability is a rate‑limited factor in phytosterols bioconversion using a cyclodextrin‑resting cell system. Catalysis Letters. 2022, 153:1557-1563
12. Jian Zhang*, Wanyuan Dong, Yuhong Ren, Dongzhi Wei. SAC‑TRAIL, a novel anticancer fusion protein: expression, purification, and functional characterization. Applied Microbiology and Biotechnology. 2022, 106(4):1511-20
13. Jian Zhang*, Kaizheng Zhang, Yuhong Ren, Dongzhi Wei. The expression, purification, and functional evaluation of the novel tumor suppressor fusion protein IL‑24‑CN. Applied Microbiology and Biotechnology. 2021, 105(20):7889-98
14. Da Wang#, Jian Zhang#, Dan-Dan Cao, Xuedong Wang*, Dongzhi Wei. Identification and in situ removal of an inhibitory intermediate to develop an efficient phytosterol bioconversion process using a cyclodextrin-resting cell system. RSC Advances. 2021, 11, 24787-93
15. Zhaoyu Chang#, Jian Zhang#, Wanyuan Dong, Xiangqi Meng, Hualei Wang, Dongzhi Wei, Yuhong Ren*. Cadmium sulfide net framework nanoparticles for photo-catalyzed cell redox. RSC Advances. 2020, 10, 37820-5
16. Zhong Wang, Jian Zhang*, Xuedong Wang*, Dongzhi Wei. High level expression and characterization of the recombinant immunotoxin DAB389-4D5 scFv targeting HER2/neu-positive ovarian carcinoma cells. Process Biochemistry. 2019, 80:26-34
17. Pengyan Jiao, Jian Zhang*, Yuguo Dong, Dongzhi Wei, Yuhong Ren*. Construction and characterization of the recombinant immunotoxin RTA-4D5-KDEL targeting HER2/neu-positive cancer cells and locating the endoplasmic reticulum. Applied Microbiology and Biotechnology. 2018, 102:9585-94
18. Jian Zhang*, Aiyou Sun*, Yuguo Dong, Dongzhi Wei. Recombinant production and characterization of SAC, the core domain of Par-4, by SUMO fusion system. Applied Biochemistry and Biotechnology. 2018, 184:1155-67
19. Jian Zhang, Rui Xu, Xinyi Tao, Yuguo Dong, Xinxin Lv, Aiyou Sun*, Dongzhi Wei*. TAT-IL-24-KDEL-induced apoptosis is inhibited by survivin but restored by the small molecular survivin inhibitor, YM155, in cancer cells. Oncotarget. 2016, 7:37030-42
20. Jian Zhang, Aiyou Sun*, Rui Xu, Xinyi Tao, Yuguo Dong, Xinxin Lv, Dongzhi Wei*. Cell-penetrating and endoplasmic reticulum-locating TAT-IL-24-KDEL fusion protein induces tumor apoptosis. Journal of Cellular Physiology. 2016, 231:84-93
21. Xinxin Lv＃, Jian Zhang＃, Rui Xu, Yuguo Dong, Aiyou Sun*, Yaling Shen, Dongzhi Wei*. Gigantoxin-4-4D5 scFv is a novel recombinant immunotoxin with specific toxicity against HER2/neu-positive ovarian carcinoma cells. Applied Microbiology and Biotechnology. 2016, 100:6403-13
22. Jian Zhang, Xinxin Lv, Rui Xu, Xinyi Tao, Yuguo Dong, Aiyou Sun*, Dongzhi Wei*. Soluble expression, rapid purification and characterization of human interleukin-24 (IL-24) using a MBP-SUMO dual fusion system in Escherichia coli. Applied Microbiology and Biotechnology. 2015, 99:6705-13
发明专利：
1. Aiyou Sun, Dongzhi Wei, Jian Zhang, Xiaojuan Wang, Chaogang Bai, Rui Xu, Yuguo Dong, Xing Zhang. The preparation and application of fusion protein TAT-IL-24-KDEL. PCT/CN2014/087669
2. 张舰,魏东芝,张子妍. 一种高产L-苏氨酸的大肠杆菌构建方法及其适应性进化.申请号2025110386302
3. 张舰,陈文欣,魏东芝. 一种变构自剪接胞嘧啶脱氨酶及其在抑癌药物中的应用.申请号2025101368907
4. 张舰,魏东芝,蒋怀谷,陈安祥. 大肠杆菌高密度发酵中提高质粒稳定性的方法.申请号202410083566.9
5. 张舰,任宇红,魏东芝,董万元.一种SAC-TRAIL融合蛋白及其制备方法和应用.申请号202110836049.0
6. 孙爱友,魏东芝,张舰,王晓娟,白超刚,徐瑞,董玉国,张星.TAT-IL-24-KDEL融合蛋白及其制备方法和应用.专利号ZL201410016426.6
张舰
上海市
研究领域：
生物医药
研究方向：
1. 重组蛋白药物制备
针对重组蛋白药物生产及应用中的关键问题，利用微生物代谢工程和合成生物学的最新技术对生产宿主和表达载体进行改造，开发高效能生产菌株。
2. 重组蛋白药物活性改造
采用理性设计方法进行药物结构改造，提高抗肿瘤药物靶向性；结合药物作用机理，实现抗肿瘤药物活性的变构调控，降低药物毒副作用，推进候选药物分子的临床前及临床研究。
3. 生物过程智能优化与调控
基于多组学大数据及微生物代谢模型，对蛋白药物和生物基产品（氨基酸类、人乳寡糖HMOs等）生产菌株的发酵过程进行智能优化与调控，建立多种宿主的发酵新工艺，实现从实验室小试优化到工厂中试以及工业化生产的一体化体系。
所在单位：
华东理工大学
