生物医药

基因编辑和表观遗传编辑
1. 基因编辑技术的开发与应用
2. 表观遗传编辑技术的开发与应用
3. CHO和293工程细胞的优化和改造

1.  Liu, Y., Lou, S., Li, J., Liu, Y., Huang, S., Wei, Y., Liu, J., Lv, R., Tang, J., Shen, Z., Sun, Y., Huang, X., Xiong, Z., Yang, H., & Zhou, C. Epigenetic editing alleviates Angelman syndrome phenotype in mice by unsilencing paternal Ube3a. Cell Discovery2024, 10: 97. https://doi.org/10.1038/s41421-024-00727-3
2.  Liu, Y., Zhou, C., Huang, S., Dang, L., Wei, Y., He, J., Zhou, Y., Mao, S., Tao, W., Zhang, Y., Yang, H., Huang, X., & Chi, T. (2020). A Cas-embedding strategy for minimizing off-target effects of DNA base editors. Nature Communications 2020, 11, 6073. https://doi.org/10.1038/s41467-020-19690-0
3.  Liu, Y., Mao, S., Huang, S., Li, Y., Chen, Y., Di, M., Huang, X., Lv, J., Wang, X., Ge, J., Shen, S., Zhang, X., Liu, D., Huang, X., & Chi, T. REPAIR x, a specific yet highly efficient programmable A > I RNA base editor. The EMBO Journal 2020, 39: e104748. https://doi.org/10.15252/embj.2020104748
4. Liu, Y., Li, J., Zhou, C., Meng, B., Wei, Y., Yang, G., Lu, Z., Shen, Q., Zhang, Y., Yang, H., & Qiao, Y. Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9). Science Bulletin 2019, 64: 1592-1600, https://doi.org/10.1016/j.scib.2019.08.023
5.  Li, G., Liu, Y., Zeng, Y., Li, J., Wang, L., Yang, G., Chen, D., Shang, X., Chen, J., Huang, X., & Liu, J. Highly efficient and precise base editing in discarded human tripronuclear embryos. Protein and Cell 2017, 8: 776–779. https://doi.org/10.1007/s13238-017-0458-7
6.  Li, X., Wang, Y., Liu, Y., Yang, B., Wang, X., Wei, J., Lu, Z., Zhang, Y., Wu, J., Huang, X., Yang, L., & Chen, J. Base editing with a Cpf1–cytidine deaminase fusion. Nature Biotechnology 2018, 36: 324-327. https://doi.org/10.1038/nbt.4102
7. Mao, S., Liu, Y., Huang, S., Huang, X., & Chi, T. (2019). Site-directed RNA editing (SDRE): Off-target effects and their countermeasures. Journal of Genetics and Genomics 2019, 46: 531–535. https://doi.org/10.1016/j.jgg.2019.11.005
8. Wei, Y., Zhou, Y., Liu, Y., Ying, W., Lv, R., Zhao, Q., Zhou, H., Zuo, E., Sun, Y., Yang, H., & Zhou, C. Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos. Protein and Cell 2021, 12: 741–745. https://doi.org/10.1007/s13238-021-00824-z
9.  Zhou, C., Sun, Y., Yan, R., Liu, Y., Zuo, E., Gu, C., Han, L., Wei, Y., Hu, X., Zeng, R., Li, Y., Zhou, H., Guo, F., & Yang, H. Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 2019.571: 275-278. https://doi.org/10.1038/s41586-019-1314-0
10.  Li, J., Shen, Z., Liu, Y., Yan, Z., Liu, Y., Lin, X., Tang, J., Lv, R., Geng, G., Xiong, Z. Q., Zhou, C., & Yang, H. A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Molecular Therapy. Molecular Therapy 2023. 31: 2286-2295. https://doi.org/10.1016/j.ymthe.2023.02.015

(1) 国家自然科学基金委员会, 青年科学基金项目, 82101552, 定点DNA甲基化成体治疗天使综合征, 2022-01-01 至 2024-12-31, 30万元, 在研, 主持