生物医药

1.体外培养环境调控干细胞扩增和分化的机制
2.干细胞体外规模化扩增和定向分化诱导技术
3.工程化人体组织体外构建新技术及生物反应器研制
4.动物细胞体外培养的无血清培养基设计和开发
5.动物细胞培养过程优化及抗体、病毒疫苗高效生产技术

1. Ziyang Jin, Yishu Zhai, Yi Zhou, Pan Guo, Miaomiao Chai, Wensong Tan, Yan Zhou*, Lian Cen*. Regulation of mesenchymal stem cell osteogenic potential via microfluidic manipulation of microcarrier surface curvature. Chemical Engineering Journal, 2022,https://doi.org/10.1016/j.cej.2022.13773.
2.  Ce Gu, Wenjing Du, Miaomiao Chai, Ziyang Jin, Yi Zhou, Pan Guo, Yan Zhou*, Wen-Song Tan. Human umbilical cord-derived mesenchymal stem cells affect urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-culture system. Biotechnology Journal. 2022, 17:2100096.
3.   Wenxia Ren, Miaomiao Chai, Mingli Jiang, Yan Zhou*, Wen-Song Tan. High glucose mediates apoptosis and osteogenesis of MSCs via downregulation of AKT-Sirt1-TWIST. Molecular Biology Reports. 2022, 49:2723-2733.
4.  Miaomiao Chai, Mingli Jiang, Ce Gu, Qiaohui Lu, Yi Zhou, Ziyang Jin, Yan Zhou*, Wen-song Tan. Osteogenically differentiated mesenchymal stem cells promote the apoptosis of human umbilical vein endothelial cells in vitro. Biotechnology and Applied Biochemistry. 2022, doi: 10.1002/bab.2274.
5. Wenjing Du, Ce Gu, Guo Pan, Yan Zhou*, Wen-Song Tan. The adverse effects of hypoxia on hiHep functions via HIF-1 alpha/PGC-1 alpha axis are alleviated by PFDC emulsion. Biochemical Engineering Journal. 2021, 176:108152.
6. Mingli Jiang, Qihua Shen, Yi Zhou, Wenxia Ren, Miaomiao Chai, Yan Zhou*, Wen-Song Tan. Fluid shear stress and endothelial cells synergistically promote osteogenesis of mesenchymal stem cells via integrin β1-FAK-ERK1/2 pathway. Turkish Journal of Biology. 2021, 45: 683-694
7. Jiazhu Xu, Qi Fang, Yanli Liu, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. In situ ornamenting poly(ε-caprolactone) electrospun fibers with different fiber diameters using chondrocyte-derived extracellular matrix for chondrogenesis of mesenchymal stem cells. Colloids and Surfaces B-Biointerfaces. 2021, 197: 111374.
8.  Chao Wei#, Bingyu Liang#, Yongsheng Li#, Bingkun Yan, Jiaqian Hou, Yan Zhou*, Yingbin Liu*, Meidong Lang*. A drug-free therapeutic system for cancer therapy by diselenide-based polymers themselves. Advanced Healthcare Materials. 2021, 10(2): e2001471.
9.  Miaomiao Chai, Ce Gu, Qihua Shen, Jiaxing Liu, Yi Zhou, Ziyang Jin, Wanli Xiong, Yan Zhou*, Wensong Tan. Hypoxia alleviates dexamethasone-induced inhibition on angiogenesis in co-culture of HUVECs and rBMSCs via HIF-1α. Stem Cell Research & Therapy. 2020, 11(1): 343.
10.  Wei Liu, Mi Zhang, Miaomiao Zhou, Ce Gu, Zhaoyang Ye, Yan Xiao*, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and evaluation of modified poly(ethylene terephthalate) microfibrous scaffolds for hepatocyte growth and functionality maintenance. Materials Science & Engineering C-Materials for Biological Applications. 2020, 109: 110523.
11. Wei Liu, Mi Zhang, Yan Xiao, Zhaoyang Ye, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds. Biochemical Engineering Journal. 2020, 160: 107565.
12. Ce Gu, Miaomiao Chai, Jiaxing Liu, Hui Wang, Wenjing Du, Yan Zhou*, Wen-Song Tan. Expansion of transdifferentiated hepatocyte in a serum-free microcarrier culture system. Digestive Diseases and Sciences. 2020, 65(7): 2009-2023.
13. Jiaxing Liu, Hui Wang, Wenxia Ren, Yan Zhou*, Wen-Song Tan. β-mercaptoethanol promotes osteogenesis of human mesenchymal stem cells via sirt1-ERK pathway. Cytotechnology. 2020, 72(5): 695-706.
14. Qihua Shen, Xiaoting Fan, Mingli Jiang, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture. Archives of Biochemistry and Biophysics. 2019, 675: 108108.
15. Huimin He, Zhaoyang Ye*, Yan Zhou*, Wensong Tan. Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask. Cell Proliferation. 2019, 2019: e12587.
16. Ce Gu, Panpan Li, Wei Liu, Yan Zhou*, Wen-Song Tan. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. Journal of Cellular and Molecular Medicine. 2019, 23(6): 4165-4178.
17. Jie Sun, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. Transforming growth factor-β1 stimulates mesenchymal stem cell proliferation by altering cell cycle through the FAK-Akt-mTOR pathway. Connective Tissue Research. 2019, 60(4): 406-417.
18. Anmin Zhou, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Bioactive Poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration. Journal of Biomaterials Applications. 2019, 33(9): 1242-1251.
19. Wei Liu, Dan Hu, Ce Gu, Yan Zhou*, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on an optimum two-stage culture strategy. Journal of Bioscience and Bioengineering. 2019, 127(4): 506-514.
20. Pan-Pan Li, Ce Gu, Bing-Yu Liang, Lin Wang, Yan Zhou*, Wen-Song Tan. A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes. Cytotechnology. 2019, 71(1): 329-344.
21. Yan Xiao*, Miaomiao Zhou, Mi Zhang, Wei Liu, Yan Zhou*, Meidong Lang*. Hepatocyte culture on 3D porous scaffolds of PCL/PMCL. Colloids and Surfaces B-Biointerfaces. 2019, 173: 185-193.
22. Mengjuan Liu, Jiaqi Wang, Hongping Tang, Li Fan, Liang Zhao, Hai-Bin Wang, Yan Zhou*, Wen-Song Tan. Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody. Biotechnology Letters. 2018, 40(11-12): 1487-1493.
23. Xiaoting Fan, Yi Teng, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs. Journal of Cell Science. 2018, 131(13): jcs216135. DOI: 10.1242/ jcs.216135.
24. Xiaoting Fan, Lei Li, Zhaoyang Ye*, Yan Zhou*, Wensong Tan. Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate. Cell Biology International. 2018, 42(4): 457-469.
25. Songjie Zhang, Min Zhou, Zhaoyang Ye *, Yan Zhou *, Wensong Tan. Fabrication of viable and functional pre-vascularized modular bone tissues by coculturing MSCs and HUVECs on microcarriers in spinner flasks. Biotechnology Journal. 2017, 12(8): 1700008.

1.  周燕, 卢巧慧, 柴苗苗, 张博韬, 谭文松. 悬浮培养用的搅拌桨及转瓶生物反应器。
2.   周燕，王照闯，郭盼，肖艳，卢巧慧，郎美东，谭文松。一种软骨培养物周期性循环压缩刺激力学装置。
3.   周燕，张咪，刘伟，谭文松一种用于肝细胞三维培养的生物支架及其制备方法和应用
4.   周燕，梁冰玉，杨晨曦，李盼盼，叶朝阳，谭文松适于肝样细胞体外培养的替代血清组合物及其使用方法
5.   周燕，吴伟，谭文松，用于骨髓间充质干细胞体外培养和扩增的无血清培养基
6.   谭文松，赵亮，周燕，黄锭适于MDCK细胞大规模贴壁培养和单细胞悬浮培养的无血清培养基
7.   谭文松，赵亮，范里，刘旭平，周燕，孙亚婷适于动物细胞高密度培养和产物表达的营养物添加剂
8.   牛红星，赵亮，范里，周燕，谭文松，适用于NS0细胞大规模培养及抗体生产的无蛋白培养基

1.国家重大研发计划：生物力学调控细胞生物学行为及微组织构建
2.国家“863”计划：（1）组织工程种子细胞体外大量扩增技术研究；（2）临床级干细胞产业化技术研发；（3）人体皮肤组织工程研究
3.国家重大新药创制：哺乳动物细胞大规模培养及药物制备关键技术
4.国家支撑计划：动物细胞大规模无血清培养及表达产品制备技术
5.国家自然科学基金：（1）微组织体外构建技术及其过程分析；（2）基于微构重塑技术构建大型预血管化工程组织
6.上海市重大科研攻关：骨髓间充质干细胞体外扩增及成骨诱导的应用研究
7.上海市基础研究重点：（1）hiHep体外规模化扩增；（2）功能性聚酯基组织工程材料制备及其对组织再生调控的研究
8.上海市科技兴农重点攻关：（1）微载体技术用于兽用细胞疫苗产业化研究；（2）猪流行性腹泻、猪传染性胃肠炎和轮状病毒的诊断及三联苗研制

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