精细化工,生物医药

发酵工程、酶催化转化等

R Cui, Z Liu*, P Yu, L Zhou，Z Zhou*. Biosynthesis of l-alanine from cis-butenedioic anhydride catalyzed by a triple-enzyme cascade via a genetically modified strain. Green Chemistry. 2021, 23, 7290. (2021)
W Hao†, W Cui†, Z Cheng, L Han, F Suo, Z Liu, L Zhou, Z Zhou*. Development of a base editor for protein evolution via in situ mutation in vivo. Nucleic Acids Research. 2021, 49, 69594–9605. (2021)
Y Liu，L Han, Z Cheng, Z Liu; Z Zhou*. Enzymatic biosynthesis of L‑2-aminobutyric acid by glutamate mutase coupled with L‑aspartate-β-decarboxylase using L‑glutamate as the sole substrate. ACS Catalysis, 2020, 10: 13913-13917 (2020)
W Cui*#, Q Lin#, R Hu, L Han, Z Cheng, Z Zhou*. Data-Driven and in Silico-Assisted Design of Broad Host-Range Minimal Intrinsic Terminators Adapted for Bacteria. ACS Synth Biol. 10(6): 1438-1450. (2021)
B Pang, L Zhou, W Cui, Z Liu, S Zhou, J Xu, Z Zhou*. A Hyperthermostable Type II Pullulanase from a Deep-Sea Microorganism Pyrococcus yayanosii CH1. J. Agric. Food Chem. 67: 9611-9617. (2019)
Y Xia, L Peplowski, Z Cheng, T Wang, Z Liu*, W Cui, M Kobayashi, Z Zhou*. Metallochaperone function of the self‐subunit swapping chaperone involved in the maturation of subunit-fused cobalt-type nitrile hydratase. Biotechnol Bioeng. 116:481–489. (2019).
Y Xia, W Cui, Z Cheng, L Peplowski, Z Liu, M Kobayashi, Z Zhou*. Improving the Thermostability and Catalytic Efficiency of the Subunit-Fused Nitrile Hydratase by Semi-Rational Engineering. Chem Cat Chem. 10:1-7. (2018)
Z Liu, L Yu, L Zhou, Z Zhou*. One-pot biosynthesis of L-aspartate from maleate via an engineered strain containing a dual-enzyme system. Appl Environ Microbiol. 85(19). pii: e01327-19. (2019)
Z Zhou, Y Hashimoto and M Kobayashi*. Self-subunit swapping chaperone needed for the maturation of multimeric metalloenzyme nitrile hydratase by a subunit exchange mechanism also carries out the oxidation of the metal ligand cysteine residues and insertion of cobalt. J Biol Chem 284, 14930-14938. (2009)
Z Zhou, Y Hashimoto, K Shiraki, and M Kobayashi. Discovery of posttranslational maturation by self-subunit swapping. Proc Natl Acad Sci USA 105, 14849-14854. (2008)

1. 周哲敏，王哲，刘中美，郭军玲，汪启；高分子量腈水合酶工程菌催化合成烟酰胺的方法 ZL201600026587.6
2. 周哲敏，刘中美，周丽，崔文璟，郭军玲，蓝瑶；一种腈水合酶突变体、含该突变体的基因工程菌及应用 ZL201910039981.3
3. Z Zhou, W Cui, Z Liu, L Zhou, Y Xia；A fused NHase with improved specific activity and stability US9896679B2
4. Z Zhou, W Cui, Z Liu, L Zhou, Y Xia；A fused NHase with improved specific activity and stability US9670474B2
5. 周哲敏;张晓欢;崔文璟；一种高效表达高分子量型腈水合酶的基因工程菌及其应用ZL201510244547.0
6. 周哲敏，崔文璟，刘中美，周丽，夏媛媛；一种比酶活和稳定性提高的融合型腈水合酶 ZL201510195795.0
7. 周哲敏，崔文璟，刘中美，周丽，夏媛媛；一种比酶活和稳定性提高的融合型腈水合酶（分案）ZL201510226690.7
8. 周哲敏，赵菡，周丽，崔文璟，刘中美，郭军玲；一种热稳定性提高的纳豆激酶 ZL201711376822.X
9. 周哲敏，庞博，周丽，崔文璟，刘中美，郭军玲，刘黎明；一种提高枯草芽孢杆菌普鲁兰酶分泌的方法 ZL201611166229.8
10. 周哲敏，刘中美，崔文璟，周丽，葛春蕾；一种新型启动子及其应用ZL201510203088.1

1. 国家重点研发计划，基于无机氮源调控的智能型霉菌表达系统的创建
2. 国家“863”计划，重组高分泌型枯草芽孢杆菌重组表达系统的开发与应用
3. 国家自然科学基金面上项目，基因融合影响腈水合酶活性和稳定性的作用机制解析
4. 国家自然科学基金面上项目，工业用腈水合酶全新蛋白质翻译后调节体系self-subunit swapping的研究
5. 教育部重大项目，谷氨酰胺转胺酶的开发及高效制备
6. 江苏省产学研项目，氨肽酶的分子改良及高效制备
7. 江苏省自然科学基金面上项目，高性能腈水合酶的构建及分子机制解析
8. 国家外专局外端高专人才项目，腈水合酶的生物合成及应用开发
9. 日本学术振兴会基础项目，放线菌Rhodococcus属微生物新型表达体系的开发和应用
10. 日本学术振兴会基础项目，霉菌的硝酸代谢新途径的解析

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