生物医药

酶学与酶工程、基因工程等领域

1、 Hao M, Cui R, Zhu X, Han L, Zhou Z, Liu Z*. Improving the activity and synergistic catalysis of L-aspartate β-decarboxylase by arginine introduction on the surface. Catalysis Science &Technology. 2022, 12:5281.
2、 Liu Y, Hao· Mingzhu, Zhou·Z, Liu Z*. Isolation and mechanism analysis of a catalytic efficiency improved L‑aspartate β‑decarboxylase toward 3‑methylaspartic acid. Systems Microbiology and Biomanufacturing. 2022, 2:157-164.
3、 Cui R, Z Liu*, P Yu, L Zhou, Z Zhou*. Biosynthesis of L-alanine from cis-butenedioic anhydride catalyzed by a triple-enzyme cascade via a genetically modified strain. Green Chemistry. 2021; 23:7290.
4、 Liu Z, Wang X, Yu J, Han L, Zhou Z*. Characterization and rational modification of aspartate 4-decarboxylase from Acinetobacter radioresistens for the production of L-alanine. Biotechnology and Bioengineering. 2021, 118:2493-250.
5、 Liu Z, Yu L, Zhou L, Zhou Z*. One-Pot Biosynthesis of L-Aspartate from Maleate via an Engineered Strain Containing a Dual-Enzyme System. Applied and Environmental Microbiology. 2019, 85:e01327-19.
6、 Liu Z, Zhao H, Han L, Cui W, Zhou L, Zhou Z*. Improvement of the acid resistance, catalytic efficiency, and thermostability of nattokinase by multisite‐directed mutagenesis. Biotechnology and Bioengineering. 2019, 116:1833-1843.

1、Method for Preparing L-Aspartic Acid with Maleic Acid by Whole-Cell Biocatalysis (US10837036B2)
2、A fused NHase with improved specific activity and stability (US9670474B2)
3、一种腈水合酶突变体、含该突变体的基因工程菌及应用（ZL2019100399813）
4、一种马来酸顺反异构酶的热稳定性改造及其应用（ZL201611108889.0）
5、一种新型启动子及其应用（一种启动子及其应用）（ZL201510203088.1）

1、国家重点研发计划（政府间国际科技创新合作重点专项）：高活性和高稳定性新型腈水合酶的开发与应用。
2、国家自然科学基金青年科学基金：纳豆激酶高效分泌表达系统的构建与最适pH偏酸性改造。
3、江苏省自然科学基金青年科学基金：枯草芽孢杆菌氨肽酶PA domain 的功能研究。

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