生物医药

工业酶制剂功能优化和高效制备等

1、Demin Kong, Lei Wang, Lingqia Su*, Jing Wu*. Effect of Leu277 on disproportionation and hydrolysis activity in Bacillus stearothermophilus NO2 cyclodextrin glucosyltransferase. Applied and Environmental Microbiology. 2021, 87(12)
2、Lingqia Su, Kailin Yao, Jing Wu*. Improved activity of Sulfolobus acidocaldarius maltooligosyltrehalose synthase through directed evolution. Journal of Agricultural and Food Chemistry, 2020, 68, 15, 4456-4463
3、Lingqia Su, Yaqi Zhao, Dan Wu, Jing Wu*. Heterogeneous expression, molecular modification of amylosucrase from Neisseria polysaccharea, and its application in the preparation of turanose. Food Chemistry. 2020, 314, 126212
4、Lingqia Su, Ruoyu Hong, Demin Kong, Jing Wu*. Enhanced activity towards polyacrylates and poly(vinyl acetate) by site-directed mutagenesis of Humicola insolens cutinase. International Journal of Biological Macromolecules, 2020, 162, 1752-1759
5、Chun Chen, Lingqia Su*, Fei Xu, Yongmei Xia, Jing Wu*. Improved Thermostability of Maltooligosyltrehalose Synthase from Arthrobacter ramosus by Directed Evolution and Site-Directed Mutagenesis. Journal of Agricultural and Food Chemistry, 2019, 67(19), 5587-5595.
6、Xiumei Tao, Lingqia Su*, Jing Wu*, Current studies on the enzymatic preparation 2-O-α-D-glucopyranosyl-L-ascorbic acid with cyclodextrin glycosyltransferase, Critical Reviews in Biotechnology, 2019, 39(2):249-257.
7、Lingqia Su, Fan Sun, Zhanzhi Liu, Kang Zhang, Jing Wu*. Highly efficient production of Clostridium cellulolyticum H10 D-psicose 3-epimerase in Bacillus subtilis and use of these cells to produce D-psicose. Microbial Cell Factories. 2018 17:188.
8、Lingqia Su, Ruoyu Hong, Xiaojie Guo, Jing Wu*, Yongmei Xia. Short-chain aliphatic ester synthesis using Thermobifida fusca cutinase. Food Chemistry. 2016, 206, 131–136.
9、Lingqia Su, Yan Huang, Jing Wu*. Enhanced production of recombinant Escherichia coli glutamate decarboxylase through optimization of induction strategy and addition of pyridoxine. Bioresource Technology. 2015, 198: 63–69.
10、Lingqia Su, Yue Ma, Jing Wu*. Extracellular expression of natural cytosolic arginine deiminase from Pseudomonas putida and its application in the production of l-citrulline. Bioresource Technology. 2015, 196: 176-183.

1、Jing Wu, Lingqia Su, Xiumei Tao, Yusheng Wang, Junchen Deng. Preparation and application of cyclodextrin glucosyltransferase mutant. US 10867099
2、Jing Wu, Lingqia Su, Zhendong Wang. Maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application Thereof. US10465177.
3、Jing Wu, Lingqia Su, Kailin Yao. A maltooligosyl trehalose synthase mutant and its application. US10273467.
4、Jing Wu, Lingqia Su, Ruoyu Hong. Methods for deinking wastepaper by combined use of cutinase and chemical reagents. US 9963824.
5、Jing Wu, Lingqia Su, and Jian Chen. A method for enhancing extracellular secretion of recombinant proteins in Escherichia coli by co-expressing Thermobifida fusca cutinase. US 9109212.
6、吴敬，宿玲恰，李玲玲，李娜. 一种降低淀粉消化率的方法及其应用. ZL201911024332.2
7、吴敬，宿玲恰，李云菲.一种用于高密度发酵的枯草芽孢杆菌. ZL201810145577.X
8、吴敬，宿玲恰，陶秀梅，汪天，赵星宇. 一种提高AA-2G产量的环糊精葡萄糖基转移酶突变体. ZL201810033866.0
9、吴敬，宿玲恰，孙帆. 一种D-阿洛酮糖 3-差向异构酶生产菌株及其固定化方法. ZL201810145983.6
10、吴敬，洪若宇，宿玲恰，陈晟. 一种利用角质酶处理造纸白水的方法. ZL201611203776.9

1、国家重点研发计划子课题，合成塑料降解酶分子改造及功能优化（2019YFA0706903），2019.12-2024.11
2、国家自然科学基金面上项目，以淀粉为底物高效制备海藻糖的酶学基础研究（31771916），2018.01-2021.12
3、国家自然科学基金青年项目，环糊精葡萄糖基转移酶催化制备2-氧-α-D-吡喃葡萄糖基-L-抗坏血酸中的关键问题研究（31501419），2016.01-2018.12
4、江苏省优秀青年基金，重组酶高效胞外表达的分子基础（BK20180082），2018.07-2021.06
5、江苏省自然科学基金，大肠杆菌重组蛋白胞外分泌的强化及优化（BK20140132），2014.07-2017.06
6、科技成果转化项目，一种提高AA-2G转化率的环糊精葡萄糖基转移酶突变体，2019.01-2021.12

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