论文著作：
1. Zeng B, Fu YS, Ye JC, Yang PH, Cui SX, Qiu WX, Li YY, Wu TX, Zhang HY, Wang YC, Du GC*, Liu S*. Ancestral sequence reconstruction of the prokaryotic three-domain laccases for efficiently degrading polyethylene. Journal of Hazardous Materials. 2024, 476: 135012
2. Aqeel SM, Abdulqader AA, Du GC*, Liu S*. Integrated strategies for efficient production of Streptomyces mobaraensis transglutaminase in Komagataella phaffii. International Journal of Biological Macromolecules. 2024, 273: 133113
3. Ye JC, Yang PH, Zhou JW, Du GC, Liu S*. Efficient production of a thermostable mutant of transglutaminase by Streptomyces mobaraensis. Journal of Agricultural and Food Chemistry. 2024, 72(8): 4207–4216.
4. Yang PH, Wang XL, Ye JC, Rao SQ, Zhou JW, Du GC, Liu S*. 2023. Enhanced thermostability and catalytic activity of Streptomyces mobaraenesis transglutaminase by rationally engineering its flexible regions. Journal of Agricultural and Food Chemistry. 71(16), 6366–6375.
5. Li YY, Li C, Aqeel SM, Wang YC, Zhang Q, Ma JL, Zhou JW, Li JH, Du GC, Liu S*. 2023. Enhanced expression of xylanase in Aspergillus niger enabling a two-step enzymatic pathway for extracting β-glucan from oat bran. Bioresource Technology. 377, 128962.
6. Xu KD, Tong Y, Li Y, Tao J, Rao SQ, Li JH, Zhou JW, Liu S*. 2023. Autoinduction AND gate inhibits cell lysis to enhance protein production in Bacillus subtilis controlled by population density and cell physiological state. ACS Synthetic Biology. 12(3), 842-851.
7. Xu KD, Tong Y, Li Y, Tao J, Rao SQ, Li JH, Zhou JW, Liu S*. 2022. Autoinduction expression modules for regulating gene expression in Bacillus subtilis. ACS Synthetic Biology. 11(12), 4220-4225
8. Song WY, Li YY, Tong Y, Li Y, Tao J, Rao SQ, Li JH, Zhou JW, Liu S*. 2022. Improving the catalytic efficiency of Aspergillus fumigatus glucoamylase toward raw starch by engineering its N‑glycosylation sites and saturation mutation. Journal of Agricultural and Food Chemistry. 70, 12672-12680.
9. Li YY, Song WY, Han XY, Wang YC, Rao SQ, Zhang Q, Zhou JW, Li JH, Liu S*, Du GC. 2022. Recent progress in key lignocellulosic enzymes: Enzyme discovery, molecular modifications, production, and enzymatic biomass saccharification. Bioresource Technology. 363, 127986.
10. Li Y, Li C, Huang H, Rao S, Zhang Q, Zhou J, Li J, Du G, Liu S*. 2022. Significantly enhanced thermostability of Aspergillus niger xylanase by modifying its highly flexible regions. Journal of Agricultural and Food Chemistry. 70 (15), 4620-4630.
11. Wang X, Du J, Zhao B, Wang H, Rao S, Du G, Zhou J, Chen J *, Liu S*. 2021. Significantly improving the thermostability and catalytic efficiency of Streptomyces mobaraenesis transglutaminase through combined rational design. Journal of Agricultural and Food Chemistry. 69(50), 15268-15278.
12. Xu KD, Tong Y, Li Y, Tao J, Rao SQ, Li JH*, Zhou JW, Liu S*. 2021. Efficient, flexible autoinduction expression systems with broad initiation in Bacillus subtilis. ACS Synthetic Biology. 10(11), 3084−3093.
13. Li C, Zhou JW, Rao SQ, Du GC*, Liu S*. 2021. Visualized multigene editing system for Aspergillus niger. ACS Synthetic Biology. 10(10), 2607–2616.
14. Yin XQ, Li YY, Zhou JW, Rao SQ, Du GC, Chen J*, Liu S*. 2021. Enhanced production of transglutaminase in Streptomyces mobaraensis through random mutagenesis and site-directed genetic modification. Journal of Agricultural and Food Chemistry. 69(10): 3144–3153.
15. Xu KD, Tong Y, Li Y, Tao J, Li JH*, Zhou JW, Liu S*. 2021. Rational design of the N-terminal coding sequence for regulating enzyme expression in Bacillus subtilis. ACS Synthetic Biology. 10(2): 265–276.
16. Li C, Zhou JW, Du GC ⁎, Chen J, Takahashi S, Liu S ⁎. 2020. Developing Aspergillus niger as a cell factory for food enzyme production. Biotechnology Advances. 44: 107630.
发明专利：
1.Song Liu, Weixin Zhao, Jian Chen, Guocheng Du. Multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins. Patent No.: US10273266B2.
2.Song Liu, Jian Chen, Guocheng Du, Weixin Zhao. Asparaginase mutant with efficient expression, activity and stability. Patent No.: US10337018B1.
3.Song Liu, Yue Feng, Jian Chen, Guocheng Du, Yun Jiao, Jie Ruan, Hongye Cheng, Hui Gao. Mutant with enhanced secretion of L-asparaginase and its application. Patent No.: US9896678B2
4.刘松;李阳阳;陈坚;堵国成.一种通过迭代饱和突变提高木聚糖酶热稳定性的方法.专利号202110952917.1,授权日期：2023年08月25日
5.刘松;王兴隆;杜建辉;周景文;陈坚;堵国成.一种催化活性和热稳定性提高的谷氨酰胺转氨酶变体.专利号:202011588017.5,授权日期：2023年08月29日
6.刘松;彭文坚;陈坚;周景文.热稳定性提高的胰蛋白酶突变体.专利号：202011184570.2,授权日期：2022年09月06日
7.刘松;李岑;堵国成;陈坚;周景文.一种多靶点编辑重组曲霉菌株的可视化筛选方法.专利号：202010847441.0，授权日期：2022年08月23日
8.刘松;尹小强;周景文;陈坚.一株重组茂原链霉菌及其在生产谷氨酰胺转氨酶中的应用.专利号：202011048198.2，授权日期：2022年07月05日
9.刘松;阮洁;冯岳;陈坚;堵国成;蕉蕴;程宏烨;高慧;吕志阳;闫沐.一种分泌能力提高的天冬酰胺酶突变体及其应用.专利号：20150837174.8，授权日期：2019年04月23日
10.刘松;陈双全;陈坚;堵国成.一种高效表达碱性果胶酶的菌株及其构建与应用.专利号：201610346038.3，授权日期：2016年05月23日
刘松
江苏省
研究领域：
生物医药
研究方向：
工业酶分子改造和食品级微生物（链霉菌、曲霉等）表达系统的开发
所在单位：
江南大学
