生物医药

酶工程与合成生物学

1、 Zhang G, Quin MB*, Schmidt-Dannert C*. Self-assembling protein scaffold system for easy in vitro coimmobilization of biocatalytic cascade enzymes. ACS Catalysis, 2018, 8:5611-5620.
2、 Zhang G, Zhao X, Li X, Du G, Zhou J*, Chen J*. Challenges and possibilities for bio-manufacturing cultured meat. Trends in Food Science & Technology, 2020, 97:443-450.
3、 Zhang G, Johnston T, Quin MB*, Schmidt-Dannert C*. Developing a protein scaffolding system for rapid enzyme immobilization and optimization of enzyme functions for biocatalysis. ACS Synthetic Biology. 2019, 8(8):1867-1876.
4、 Zhang G*, Chen Y, Li Q, Zhou J, Li J, Du G. Growth-coupled evolution and high-throughput screening assisted rapid enhancement for amylase-producing Bacillus licheniformis. Bioresource Technology, 2021, 337: 125467.
5、 Zhang G*, Ma S, Liu X, Yin X, Zhou J, Li J, Du G. Protein-glutaminase: research progress and prospect in food manufacturing. Food Bioscience, 2021, 43:101314.
6、 Zhang G, Schmidt-Dannert S, Quin MB*, Schmidt-Dannert C*. Protein-based scaffolds for enzyme immobilization. Methods Enzymol. 2019, 617:323-362.
7、 Zhang G, Lin Y, Qi X, Li L, Wang Q*, Ma Y. TALENs assisted multiplex editing for accelerated genome evolution in Saccharomyces cerevisiae. ACS Synthetic Biology, 2015, 4(10):1101-1111.
8、 Zhang G, Wang Q*. Development, characterization and biomanufacturing of sugar alcohols, Functional Carbohydrates. 2017, pp. 269-290, CRC Press.
9、 Zhang G, Chen Y, Du G*. Advances in enzyme catalysis and technologies: protein engineering approaches for enhanced catalytic efficiency. Biomass, Biofuels, Biochemicals, 2020, pp.103-117, Elsevier.
10、 Pang C, Liu S, Zhang G*, Zhou J, Du G, Li J. Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli. Biotechnology for Biofuels and Bioproducts, 2022, 15.
11、 Li Q, Lu J, Zhang G*, Liu S, Zhou J, Du G, Chen J. Recent advances in the development of Aspergillus for protein production. Bioresource Technology, 2022, 348:126768.
12、 Pang C, Liu S, Zhang G*, Zhou J, Du G, Li J. Enhancing extracellular production of lipoxygenase in Escherichia coli by signal peptides and autolysis system. Microbial Cell Factories, 2022, 21(1): 42.
13、 Pang C, Liu S, Zhang G*, Zhou J, Du G, Li J. Combinatorial strategy towards the efficient expression of lipoxygenase in Escherichia coli at elevated temperatures. Applied Microbiology and Biotechnology, 2021, 104(23):10047-10057.
14、 Pang C, Liu S, Zhang G*, Zhou J, Du G, Li J. Improving the catalytic efficiency of Pseudomonas aeruginosa lipoxygenase by semi-rational design. Enzyme and Microbial Technology, 2022, 162:110120.
15、 Yin X, Zhang G*, Ma S, Zhou J, Li J, Du G*. Combinatorial engineering for efficient production of protein-glutaminase in Bacillus subtilis. Enzyme and Microbial Technology, 2021, 150: 109863.
16、 Pang C, Yin X, Liu S, Zhang G*, Liu S, Zhou J, Li J*, Du G. Current progress and prospects of enzyme technologies in future foods. Systems Microbiology and Biomanufacturing, 2021, 1:24-32.

1、 张国强，堵国成，殷鑫鑫，周景文，陈坚，李江华。一种提高蛋白质谷氨酰胺酶分泌表达的方法，专利号：202110132935.5
2、 张国强，陈坚，堵国成，李江华，周景文。一种α-氨基酸酯酰基转移酶在细胞表面表达的重组大肠杆菌与应用，专利号：202111339299.X
3、 张国强，堵国成，陈帅丽，周景文，李江华，陈坚。一种温度控制表达系统与应用，专利号：202011521964.2
4、 张国强，刘松，李江华，庞翠萍，周景文，堵国成。一种催化效率与热稳定性提高的脂肪氧合酶突变体，专利号：202111625967.5
5、 张国强，周景文，滕茂放，李江华，堵国成，陈坚。一种诱导型启动子的进化方法及其应用，专利号：2021103088461
6、 王钦宏，张国强，贺鹏。一种新型产糖醇酵母菌株基因组重排技术的构建及其应用，专利号：201510070962.9
7、 张国强，李江华，沈秀茹，陈坚，堵国成。一株产中性乳糖酶的乳酸克鲁维酵母，专利号：202210609797.X

1、 蛋白质谷氨酰胺酶在枯草芽孢杆菌中的激活调控机制研究，国家自然科学基金面上项目，2022-2025
2、 微生物化学品工厂的途径创建及应用，国家重点研发计划子课题， 2019-2024
3、 纺织酶制剂制备与应用技术及产业示范，国家重点研发计划子课题，2022-2027
4、 蛋白质功能设计与高效制造的核心技术基础，江苏省前沿引领技术基础研究专项子课题，2020-2025
5、 产中性乳糖酶乳酸克鲁维酵母菌株的筛选与表达机制解析，青岛市生物制造行业智库联合基金，2021-2022，
6、 体内连续定向进化对α-淀粉酶耐酸性改造的研究，江苏省自然科学青年基金，2019-2022
7、 高产蛋白质谷氨酰胺酶菌株的构建与发酵优化，企业合作，2021-2022
8、 脂肪酶底物特异性分子改造研究，企业合作，2019-2023

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