生物医药

1. RNA与DNA修饰相关蛋白的催化、识别、代谢机制研究；
2. 非编码RNA生成相关蛋白的结构与功能；
3. DNA复制与损伤修复相关蛋白的结构生物学研究；
4. 环状RNA疫苗研究；
5. 天然代谢产物生成的酶学机制研究。

1) Meng, C. #, Guo, W. #, Xiao, C. #, Wen, Y. #, Zhu, X., Zhang, Q., Liang, Y., Li, H., Xu, S., Qiu, Y. *, Chen, H. *, Lin, WJ. *, Wu, B.* (2025) Structural Basis for Psilocybin Biosynthesis. Nature Communications.
2) Li, X. #, Guo W. #, Wen, Y. #, Meng, C., Zhang, Q., Chen, H., Zhao, X.*, Wu, B.* (2025) Molecular basis for the RNA binding properties of IGF2BP3 tandem RBDs. Structure. 2025 Feb 12: S0969-2126(25)00022-X.
3) Wen, Y.#, Guo, W.#, Meng, C., Yang, J., Xu, S., Chen, H.*, Gan, J.*, Wu, B.*. Structural insights into the biosynthetic mechanism of Nα-GlyT and 5-NmdU hypermodifications of DNA. Nucleic Acids Research. 2024 Sep 12: gkae784.
4) Guo, W.#, Wu, W.#, Wen, Y., Gao, Y., Zhuang, S., Meng, C., Chen, H., Zhao, Z.*, Hu, K.*, Wu, B.*. Structural insights into the catalytic mechanism of the AP endonuclease AtARP. Structure. 2024. 32, 1-15
5) Guo, W.#; Li, X.#; Fan, J.#; Li, H.#; Wen, Y.; Meng, C.; Zhu, X.; Chen, H.; Zhang, Y.; Du, Y.*; Wu, B.*, Structural characterization of an isocytosine-specific deaminase VCZ reveals its application potential in the anti-cancer therapy. iScience. 2023, 26(9):107672
6) Liu, D.; Dredge, B. K.; Bert, A.; Pillman, K.; Toubia, J.; Guo, W.; Dyakov, B. J.A.; Migault, M.; Conn, V. M.; Conn, S. J.; Gregory, P. A.; Gingras, A-C; Patel, D.; Wu, B.*; Goodall, G.*, ESRP1 controls biogenesis and function of a large abundant multiexon circRNA. Nucleic acids research. 2024 Feb 9;52(3):1387-1403.
7) Li, X. #, Li, K. #, Guo, W., Wen, Y., Meng, C., Wu, B.*. (2022) Structure characterization of E. coli pseudouridine kinase PsuK. Frontiers in Microbiology. 2022; 13:926099.
8) Wen Y.#, Li X.#, Guo W, Wu B*. Crystal structures of a new class of pyrimidine/purine nucleoside phosphorylase revealed a Cupin fold. Proteins. 2022; 90(6):1233-1241.
9) Liu Y.#, Zhang L.#, Guo M., Chen L., Wu B*, Huang H.*. Structural basis for anti-CRISPR repression mediated by Aca1 and Aca2. 2021. Journal of Biological Chemistry. Dec;297(6):101357.
10) Hou, Y.#, Sun J.#, Wu, B.#, Gao, Y.#, Nie H., Nie, Z., Quan, S., Wang Y.*, Cao, X*, Li, S.* (2021) CPSF30-L-mediated recognition of mRNA m6A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis. Molecular Plant. 2021 Apr 5;14(4):688-699.
11) Wang, N.#, Bao, H.#, Chen, L., Liu, Y., Li, Y., Wu, B.*, Huang H.* Molecular basis of abasic site sensing in single-stranded DNA by the SRAP domain of E. coli yedK. Nucleic acids research. 2019 Nov 4;47(19):10388-10399.
12) Wu, B.#,*, Zhang, D.#, Nie, H.#, Shen, S., Li, Y., Li, S.* (2019) Structure of Arabidopsis thaliana N6-methyl-AMP deaminase ADAL with bound GMP and IMP and implications for N6-methyl-AMP recognition and processing, RNA Biology, 16:10, 1504-1512
13) Qiu, Q.#, Mei, H.#, Deng, X.#, He, K.#, Wu, B.#, Yao, Q., Zhang J., Lu, F., Ma, J.*, Cao, X.*(2019) DNA methylation repels targeting of Arabidopsis REF6. Nature Communications. (2019) 10:2063
14) Wu, B. #, Su, S. #, Deepak P. Patil, Liu, H., Gan, J., Samie R. Jaffrey, Ma, J.* (2018) Molecular Basis for The Specific and Multivariate Recognitions of RNA Substrates by Human hnRNPA2/B1. Nature Communications. 9(1):420.
15) Wu, B., Li, L., Huang, Y*., Ma, J*., Min J*. (2017). Readers, writers and erasers of N6-methylated adenosine modification, Current Opinion in Structural Biology. 47:67-76.
16) Li, W.#, Wu, B.#, Soca, WA., An, L.* (2018) Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-terminal Domain. Journal of Virology. 92(14). pii: e00324-18.
17) Wu, B. *, Xu, J., Su, S., Liu, H., Gan, J., Ma, J.* (2017) Structural insights into the specific recognition of DSR by the YTH domain-containing protein Mmi1. Biochemical and Biophysical Research Communications. 491(2):310-316.
18) Li, Y.#, Wu, B.#, Liu, H., Gao, Y., Yang C., Chen X., Zhang, J., Chen, Y., Gu, Y., Li, J., Ma, J., Gan, J.* (2018) Structural insights into target DNA recognition by the double homeodomains of human DUX4. Biochemical and Biophysical Research Communications. 505(4):1161-1167
19) Wu, B.#,*, Zhang, M.#, Su, S., Liu, H., Gan, J., Ma, J.* (2018) Structural insight into the role of VAL1 for targeting to FLC locus in Arabidopsis thaliana. Biochemical and Biophysical Research Communications. 501(2):415-422

[1] 国家自然科学基金；批准年度：2018年；项目起止年月：2018-01至2021-12（已结题，参与）
[2] 中山大学“百人计划”启动项目；批准年度：2020年；项目起止年月：2020-06至2023-06（主持）
[3] 国家自然科学基金青年科学基金项目；批准年度：2019年；项目起止年月：2020-01至2021-12（已结题，主持）
[4] 广东省恶性肿瘤表观遗传与基因调控重点实验室开放课题专项基金；批准年度：2021年；项目起止年月：2021-02至2022-12 (已结题，主持)
[5] 中山大学青年教师培育项目（重点项目）；2023年；项目起止年月：2023-08至2023-12 (已结题，主持)
[6] 广东省卫健委“特支计划”青年拔尖人才项目；2024年；项目起止年月：2024-10至2027-10 (在研，主持)