新材料,石油炼制,石油化工,精细化工,生物医药

酶及纳米酶；智能催化材料；磁性纳米材料；疾病诊断和精准治疗

O-glcnac;General;Sialic Acid;Glycoside Hydrolase;Glycosylation;Carbohydrate-active Enzyme;Enzyme;Inhibitor;Antimicrobial Resistance;Isothermal Titration Calorimetry

Zheng, L., Zhang, Y., Shi, R., Xue, X., Li, K., Zhang, W., Qiang, J., Peng, M., He, Y. *, Fan, H.M. * (2025). Nanohybrid urate oxidase with magnetically switchable catalytic potential for precise gout therapy. Biomaterials, 320: 123277.

Zhang, T. B., Li, J., Lu, J. J., Li, J. W., Zhang, H., Miao, Y. Q., Liu, X. L., He, Y.*, Yang, L., & Fan, H. M.* (2025). Enhanced tumor-targeting ability of transferrin-functionalized magnetic nanoparticles by AMF stimulation. Biomaterials, 315. https://doi.org/ARTN 12292510.1016/j.biomaterials.2024.122925

Peng, J. C., Wang, N. N., Xu, R., Fan, H. M.*, & He, Y.* (2024). Advancing DNAzyme Technology in Tumor Treatment. Catalysts, 14(11). https://doi.org/ARTN 79510.3390/catal14110795

Li, J. Q., Rui-Xing, S., Xu, J. Y., Zheng, L., Wang, N. N., Li, G. L., Fan, H. M., & He, Y.* (2024). Research Progress and Biomedical Applications of Magneto-controlled Nanobiocatalysis. Progress in Biochemistry and Biophysics, 51(12), 3123-3135. 李佳琪,石瑞兴,徐佳瑶,郑璐,王妮妮,李嘎龙,樊海明,和媛.磁场调控纳米生物催化的研究进展与生物医学应用[J].生物化学与生物物理进展,2024,51(12):3123-3135 https://doi.org/10.16476/j.pibb.2024.0158

Dong, M. C., Chen, Z. Y., He, Y., Zallot, R., & Jin, Y. (2024). Bioinformatics-Facilitated Identification of Novel Bacterial Sulfoglycosidases That Hydrolyze 6-Sulfo--acetylglucosamine. Acs Bio & Med Chem Au, 4(6), 342-352. https://doi.org/10.1021/acsbiomedchemau.4c00088

Wang, Y., Wang, X., Dong, Y. H., Peng, M. L., Guo, L. N., Cui, M. Y., He, Y., Yi, J. B., Ma, H. J., Zhang, H., & Fan, H. M. (2024). Magnetic-responsive Pickering emulsions based on MFeO (M = Mn, Fe, Co, Ni, Cu, Zn) for green and efficient oxidation of benzyl alcohol. Green Chemistry, 26(10), 6131-6138. https://doi.org/10.1039/d4gc00554f

Zhou, Q., Catalán, P., Bell, H., Baumann, P., Cooke, R., Evans, R., Yang, J., Zhang, Z., Zappalà, D., Zhang, Y., Blackburn, G. M., He, Y. *, & Jin, Y. * (2023). An Ion-Pair Induced Intermediate Complex Captured in Class D Carbapenemase Reveals Chloride Ion as a Janus Effector Modulating Activity. ACS Cent. Sci., 2023. 9, 12, 2339–2349. https://doi.org/doi.org/10.1021/acscentsci.3c00609

Zhang, Z., Dong, M. C., Zallot, R., Blackburn, G. M., Wang, N. N., Wang, C. J., Chen, L., Baumann, P., Wu, Z. Y., Wang, Z. F., Fan, H. M., Roth, C., Jin, Y. *, & He, Y. * (2023). Mechanistic and Structural Insights into the Specificity and Biological Functions of Bacterial Sulfoglycosidases. ACS Catal, 13, 824-836. https://doi.org/10.1021/acscatal.2c05405

He, Y., Chen, X., Zhang, Y., Wang, Y., Cui, M., Li, G., Liu, X., & Fan, H.* (2022). Magnetoresponsive nanozyme: magnetic stimulation on the nanozyme activity of iron oxide nanoparticles. Sci China Life Sci, 65(1), 184-192. https://doi.org/10.1007/s11427-020-1907-6

Jiao, W., Zhang, T., Peng, M., Yi, J., He, Y. *, & Fan, H.* (2022). Design of Magnetic Nanoplatforms for Cancer Theranostics. Biosensors, 12(1). https://doi.org/10.3390/bios12010038

Zhang, H., Guo, Y., Jiao, J., Qiu, Y., Miao, Y., He, Y., Li, Z., Xia, C., Li, L., Cai, J., Xu, K., Liu, X., Zhang, C., Bay, B. H., Song, S., Yang, Y., Peng, M., Wang, Y., & Fan, H. (2022). A hepatocyte-targeting nanoparticle for enhanced hepatobiliary magnetic resonance imaging. Nat Biomed Eng, 7(3), 221-235. https://doi.org/10.1038/s41551-022-00975-2

Liu, X. L., Zhang, Y. F., Guo, Y., Jiao, W. B., Gao, X., Lee, W. S. V., Wang, Y. Y., Deng, X., He, Y., Jiao, J., Zhang, C., Hu, G. Q., Liang, X. J., & Fan, H. M. (2021). Electromagnetic Field-Programmed Magnetic Vortex Nanodelivery System for Efficacious Cancer Therapy. Adv Sci, 8(18), e2100950. https://doi.org/10.1002/advs.202100950

Zhang, T. B., Li, G. L., Miao, Y. Q., Lu, J. J., Gong, N. Q., Zhang, Y. F., Sun, Y. T., He, Y., Peng, M. L., Liu, X. L., Liang, X. J., & Fan, H. M. (2021). Magnetothermal regulation of in vivo protein corona formation on magnetic nanoparticles for improved cancer nanotherapy. Biomaterials, 276, 121021. https://doi.org/10.1016/j.biomaterials.2021.121021.

Zhang, Y., Wang, Y., Zhou, Q., Chen, X., Jiao, W., Li, G., Peng, M., Liu, X., He, Y.*, & Fan, H. * (2021). Precise Regulation of Enzyme-Nanozyme Cascade Reaction Kinetics by Magnetic Actuation toward Efficient Tumor Therapy. ACS Appl Mater Interfaces, 13(44), 52395-52405. https://doi.org/10.1021/acsami.1c15717

Lei, J. E., Wang, Q., Lin, Y., Li, F., Ma, C., He, Y.*, & Xu, J. R.* (2020). Rapid detection of extended-spectrum beta-Lactamases producers in Enterobacteriaceae using a calorimetry approach. J Appl Microbiol, 130, 1523-1530. https://doi.org/10.1111/jam.14841

Wasey, A., Yang, J., Sun, D., He, Y.*, & Zhang, C.* 2020. On-chip Carba NP test for accurate and high throughput detection of carbapenemase-producing Enterobacteriaceae. Talanta, 210, 120656. doi: 10.1016/j.talanta.2019.120656

Liu, X., Yan, B., Li, Y., Ma, X., Jiao, W., Shi, K., Zhang, T., Chen, S., He, Y., Liang, X. J., & Fan, H. 2020. Graphene Oxide-Grafted Magnetic Nanorings Mediated Magnetothermodynamic Therapy Favoring Reactive Oxygen Species-Related Immune Response for Enhanced Antitumor Efficacy. ACS Nano, 14(2), 1936-1950. doi: 10.1021/acsnano.9b08320

Liu, X., Peng, M., Li, G., Miao, Y., Luo, H., Jing, G., He, Y., Zhang, C., Zhang, F., & Fan, H. 2019. Ultrasonication-Triggered Ubiquitous Assembly of Magnetic Janus Amphiphilic Nanoparticles in Cancer Theranostic Applications. Nano Lett, 19(6), 4118-4125. doi: 10.1021/acs.nanolett.9b01524


Peng, X., Wang, B., Yang, Y., Zhang, Y., Liu, Y., He, Y., Zhang, C., & Fan, H. 2019. Liver Tumor Spheroid Reconstitution for Testing Mitochondrial Targeted Magnetic Hyperthermia Treatment. ACS Biomater Sci Eng, 5(3), 1635-1644. doi: 10.1021/acsbiomaterials.8b01630

Xiong, R., Zhang, W., Zhang, Y., Zhang, Y., Chen, Y., He, Y.*, & Fan, H.* 2019. Remote and real time control of an FVIO-enzyme hybrid nanocatalyst using magnetic stimulation. Nanoscale, 11(39), 18081-18089. doi: 10.1039/c9nr04289j

Wang, Q., He, Y.*, Lu, R., Wang, W. M., Yang, K. W.*, Fan, H. M., Jin, Y., & Blackburn, G. M. 2018. Thermokinetic profile of NDM-1 and its inhibition by small carboxylic acids. Biosci Rep, 38(2). doi: 10.1042/BSR20180244

Xiang, Y., Chen, C., Wang, W. M., Xu, L. W., Yang, K. W.*, Oelschlaeger, P., & He, Y.* 2018. Rhodanine as a Potent Scaffold for the Development of Broad-Spectrum Metallo-beta-lactamase Inhibitors. ACS Med Chem Lett, 9(4), 359-364. doi: 10.1021/acsmedchemlett.7b00548

Zhang, H., Liu, X. L., Zhang, Y. F., Gao, F., Li, G. L., He, Y., Peng, M. L., & Fan, H. M. 2018. Magnetic nanoparticles based cancer therapy: current status and applications. Sci China Life Sci, 61(4), 400-414. doi: 10.1007/s11427-017-9271-1

Zhang, Y., Lei, J. E., He, Y.*, Yang, J., Wang, W., Wasey, A., Xu, J.*, Lin, Y., Fan, H., Jing, G., Zhang, C., & Jin, Y. 2018. Label-Free Visualization of Carbapenemase Activity in Living Bacteria. Angew Chem Int Ed, 57(52), 17120-17124. doi: 10.1002/anie.201810834

Li, G., Ma, P., He, Y., Zhang, Y., Luo, Y., Zhang, C., & Fan, H.* 2018. Enzyme-Nanowire Mesocrystal Hybrid Materials with an Extremely High Biocatalytic Activity. Nano Lett, 18(9), 5919-5926. doi: 10.1021/acs.nanolett.8b02620

Wang, W. J., Wang, Q., Zhang, Y., Lu, R., Zhang, Y. L., Yang, K. W.*, Lei, J. E., & He, Y. * 2017. Characterization of beta-lactamase activity using isothermal titration calorimetry. Biochim Biophys Acta Gen Subj, 1861(8), 2031-2038. doi: 10.1016/j.bbagen.2017.04.011

Cekic, N., Heinonen, J. E., Stubbs, K. A., Roth, C., He, Y., Bennet, A. J., McEachern, E. J., Davies, G. J., & Vocadlo, D. J. 2016. Analysis of transition state mimicry by tight binding aminothiazoline inhibitors provides insight into catalysis by human O-GlcNAcase. Chem Sci, 7(6), 3742-3750. doi: 10.1039/c6sc00370b

Macauley, M. S., Kawasaki, N., Peng, W., Wang, S.-H., He, Y., Arlian, B. M., McBride, R., Kannagi, R., Khoo, K.-H., & Paulson, J. C. 2015. Unmasking of CD22 Co-receptor on Germinal Center B-cells Occurs by Alternative Mechanisms in Mouse and Man. J Biol Chem, 290(50), 30066-30077. doi: 10.1074/jbc.M115.691337

Rillahan, C. D., Macauley, M. S., Schwartz, E., He, Y., McBride, R., Arlian, B. M., Rangarajan, J., Fokin, V. V., & Paulson, J. C. 2014. Disubstituted Sialic Acid Ligands Targeting Siglecs CD33 and CD22 Associated with Myeloid Leukaemias and B Cell Lymphomas. Chem Sci, 5(6), 2398-2406. doi: 10.1039/C4SC00451E

He, Y., Roth, C., Turkenburg, J. P., & Davies, G. J. 2014. Three-dimensional structure of a Streptomyces sviceus GNAT acetyltransferase with similarity to the C-terminal domain of the human GH84 O-GlcNAcase. Acta Crystallogr D Biol Crystallogr, 70(Pt 1), 186-195. doi: 10.1107/S1399004713029155

Darby, J. F., Landstrom, J., Roth, C., He, Y., Davies, G. J., & Hubbard, R. E. 2014. Discovery of selective small-molecule activators of a bacterial glycoside hydrolase. Angew Chem Int Ed, 53(49), 13419-13423. doi: 10.1002/anie.201407081

Macauley, M. S., Chan, J., Zandberg, W. F., He, Y., Whitworth, G. E., Stubbs, K. A., Yuzwa, S. A., Bennet, A. J., Varki, A., Davies, G. J., & Vocadlo, D. J. 2012. Metabolism of vertebrate amino sugars with N-glycolyl groups: intracellular beta-O-linked N-glycolylglucosamine (GlcNGc), UDP-GlcNGc, and the biochemical and structural rationale for the substrate tolerance of beta-O-linked beta-N-acetylglucosaminidase. J Biol Chem, 287(34), 28882-28897. doi: 10.1074/jbc.M112.363721

He, Y., Bubb, A. K., Stubbs, K. A., Gloster, T. M., & Davies, G. J. 2011. Inhibition of a bacterial O-GlcNAcase homologue by lactone and lactam derivatives: structural, kinetic and thermodynamic analyses. Amino Acids, 40(3), 829-839. doi: 10.1007/s00726-010-0700-6

He, Y., Macauley, M. S., Stubbs, K. A., Vocadlo, D. J., & Davies, G. J. 2010. Visualizing the reaction coordinate of an O-GlcNAc hydrolase. J Am Chem Soc, 132(6), 1807-1809. doi: 10.1021/ja9086769

Martinez-Fleites, C., He, Y., & Davies, G. J. 2010. Structural analyses of enzymes involved in the O-GlcNAc modification. Biochim Biophys Acta, 1800(2), 122-133. doi: 10.1016/j.bbagen.2009.07.019

Macauley, M. S., He, Y., Gloster, T. M., Stubbs, K. A., Davies, G. J., & Vocadlo, D. J. 2010. Inhibition of O-GlcNAcase using a potent and cell-permeable inhibitor does not induce insulin resistance in 3T3-L1 adipocytes. Chem Biol, 17(9), 937-948. doi: 10.1016/j.chembiol.2010.07.006

Marcelo, F., He, Y., Yuzwa, S. A., Nieto, L., Jimenez-Barbero, J., Sollogoub, M., Vocadlo, D. J., Davies, G. D., & Bleriot, Y. 2009. Molecular basis for inhibition of GH84 glycoside hydrolases by substituted azepanes: conformational flexibility enables probing of substrate distortion. J Am Chem Soc, 131(15), 5390-5392. doi: 10.1021/ja809776r

He, Y., Martinez-Fleites, C., Bubb, A., Gloster, T. M., & Davies, G. J. 2009. Structural insight into the mechanism of streptozotocin inhibition of O-GlcNAcase. Carbohydr Res, 344(5), 627-631. doi: 10.1016/j.carres.2008.12.007

Balcewich, M. D., Stubbs, K. A., He, Y., James, T. W., Davies, G. J., Vocadlo, D. J., & Mark, B. L. 2009. Insight into a strategy for attenuating AmpC-mediated beta-lactam resistance: structural basis for selective inhibition of the glycoside hydrolase NagZ. Protein Sci, 18(7), 1541-1551. doi: 10.1002/pro.137

Martinez-Fleites, C., Macauley, M. S., He, Y., Shen, D. L., Vocadlo, D. J., & Davies, G. J. 2008. Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation. Nat Struct Mol Biol, 15(7), 764-765. doi: 10.1038/nsmb.1443

Yuzwa, S. A., Macauley, M. S., Heinonen, J. E., Shan, X., Dennis, R. J., He, Y., Whitworth, G. E., Stubbs, K. A., McEachern, E. J., Davies, G. J., & Vocadlo, D. J. 2008. A potent mechanism-inspired O-GlcNAcase inhibitor that blocks phosphorylation of tau in vivo. Nat Chem Biol, 4(8), 483-490. doi: 10.1038/nchembio.96

Wang, Z.-F., He, Y., & Huang, L.-J. 2007. An alternative method for the rapid synthesis of partially O-methylated alditol acetate standards for GC-MS analysis of carbohydrates. Carbohydr Res, 342(14), 2149-2151. doi: 10.1016/j.carres.2007.05.028

·一种β-内酰胺类抗生素敏感性的快速检测方法，2019，专利号：ZL201611185195.7
·一种针对产苯唑西林酶OXA-48细菌的特异性检测方法，2020，专利号：ZL201811259265.8

·针对OGA糖苷酶的新型活性片段筛选与生物活性分析研究，31400663，国家级，2015-01~2017-12
·新型小分子活化剂对人N-乙酰葡糖胺水解酶活性调控的研究，2014JQ3090，省部级，2014-01~2015-12
·新型小分子活化剂对人N-乙酰葡糖胺水解酶活性调控的研究，14JK1764，省部级，2014-01~2015-12
·基于细菌毒素新型O-GlcNAc 糖基转移反应的分子识别和催化机理研究，2019KW-078，省部级，2019-01~2020-12
·抗生素耐药靶蛋白金属-内酰胺酶的抑制研究，81361138018，国家级，2014-01~2015-12
·酶活动态可调的磁响应纳米杂化尿酸酶的构建及其体内抗痛风性能研究，32371456，国家级，2024-01~2027-12

技术咨询