精细化工

1. 重要核苷类功能分子(侧重天然药物)的生物合成
基于基因组大数据，高效发掘具有自主知识产权的重要核苷类功能分子(聚焦但不局限)及相关基因(酶)资源，积极参与国际竞争；与此同时，汇聚多学科研究手段，对活性显著、结构独特的重要天然药物(生物功能分子)的生物合成机理进行探究，以破解其生物合成过程中神奇的化学与生物学之谜。
2. 重要核苷类功能分子的生物智造
以创造更卓越“大健康”产品、突破“卡脖子”技术为奋斗目标，实现重要核苷类功能分子(药物及其中间体、精细化学品与食品添加剂/功能因子等)的高效生物智造，进而提升相关产业的技术水平与国际竞争力；此外，与著名生物制药企业深度合作，系统解析目标药物的合成途径与代谢网络，并综合合成生物学策略，以定向提高目标药物(有效组分)的产量和质量，从而直接服务于工业化生产。
3. 重要核苷类(不局限于)功能分子的生理功能与生物代谢
聚焦大健康科学问题，以“基础-应用”的多学科深度合作为驱动力，从分子水平上揭示核苷类功能分子的生理功能与代谢过程；此外，锚定医学科学前沿，以揭示核苷类生物功能分子的代谢与防御机制为突破口，进而为相关疾病的发病机制与临床治疗提供理论指导与科学依据。

1. Gong R#，Qu Y#，Li J#, Zhang X, Zhou L, Tian Z, Zeng X, Jin B, Li Z, Yu L, Chen R, Zhou Y, Liao L, Yang L, Song X, Cai Y-S, Shen K, Deng Z, Zhang Z*, Wang B*, Chen W*. (2025) A two-metalloenzyme cascade constructs the azetidine-containing pharmacophore, Nat Chem. DOI: 10.1038/s41557-025-01949-y. (*共通讯作者)
2. Yu L#, Chen R#, Zhang C#, Wang Z, Wang Z, Zeng X, Liang H, Wang Z, He Y, She Y, Wang Y, Gong R, Song X, Deng Z, Fei Q, Chen W*. (2025) A combined pseudouridine biomanufacturing platform enabled by a streamlined designer pathway. Nat Commun. 16(1):8866. (*通讯作者)
3. Wang Z#, Li J#, Wang X#, Jin B, Zhou L, Zhao Z, Gu M, Wang J, Song X, Deng Z, Wu S*, Zhang Z* Chen W*. (2025) Efficient synthesis of glycodiversified nucleoside analogues by a thermophilic promiscuous glycosyltransferase, ACS Catal.15:1217−1229 (*共通讯作者)
4. Yu L#, Gao Y#, He Y, Liu Y, Shen J, Liang H, Gong R, Duan H, Price NPJ, Song X, Deng Z, Chen W*. (2024) Developing the E. coli platform for efficient production of UMP-derived chemicals. Metab Eng. 24(83):61-74. (*通讯作者)
5. Zhai G#, Gong R#, Lin Y, Zhang M, Li J, Deng Z, Sun J, Chen W*, Zhang Z*. (2022) Structural insight into the catalytic mechanism of non-heme iron halogenase AdaV in 2′-chloropentostatin biosynthesis. ACS Catal. 12:13910-13920. (*共通讯作者)
6. Zhang M#, Kong L#, Gong R#, Iorio M, Donadio S, Deng Z, Sosio M, Chen W*. (2022). Biosynthesis of C-nucleoside antibiotics in actinobacteria: recent advances and future developments. Microb Cell Fact. 21(1):2. (*通讯作者)
7. Yu L#, Zhou W#, She Y#, Ma H, Cai Y-S, Jiang M, Deng Z, Price NPJ*, Chen W*. (2021) Efficient biosynthesis of nucleoside cytokinin angustmycin A containing an unusual sugar system. Nat Commun. 12(1):6633. (*共通讯作者)
8. Gong R#, Yu L#, Qin Y, Price NPJ, He X, Deng Z*, Chen W*. (2021) Harnessing synthetic biology-based strategies for engineered biosynthesis of nucleoside natural products in actinobacteria. Biotechnol Adv, 46:107673. (*共通讯作者)
9. Zhang M#, Zhang P#, Xu G#, Zhou W, Gao Y, Gong R, Cai Y-S, Cong H, Deng Z, Price NPJ, Mao X*, Chen W*. (2020) Comparative investigation into formycin A and pyrazofurin A biosynthesis reveals branch pathways for the construction of C-nucleoside scaffolds. Appl Environ Microbiol. 86(2). (*共通讯作者)
10. Kong L#, Xu G#, Liu X#, Wang J, Tang Z, Cai Y-S, Shen K, Tao W, Zheng Y, Deng Z, Price NPJ, Chen W*. (2019) Divergent biosynthesis of C-nucleoside minimycin and indigoidine in bacteria. iScience (Cell子刊), 22:430-440. (*通讯作者)(Highlighted in ASM Blog)
11. Xu G, Kong L, Xu L, Gao Y, Jiang M, Cai Y-S, Hong K, Hu Y, Liu P, Deng Z, Price NPJ, Chen W*. (2018) Coordinated biosynthesis of the purine nucleoside antibiotics aristeromycin and coformycin in Actinomycetes. Appl Environ Microbiol. 84(22). (*通讯作者)
12. Liu Y, Gong R, Liu X, Zhang P, Zhang Q, Cai Y-S, Deng Z, Winkler M, Wu J*, Chen W*. (2018) Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090. Microb Cell Fact. 17:131. (*共通讯作者)
13. Gong R, Qi J, Wu P, Cai Y-S, Ma H, Liu Y, Duan H, Wang M, Deng Z, Price NPJ, Chen W*. (2018) An ATP-dependent ligase with substrate flexibility involved in assembly of the peptidyl nucleoside antibiotic polyoxin. Appl Environ Microbiol. 84(13). (*通讯作者)
14. Gao Y#, Xu G#, Wu P#, Liu J, Deng Z, Chen W*. (2017) Biosynthesis of 2′-chloropentostatin and 2′-amino-2′-deoxyadenosine highlights a single gene cluster responsible for two individual pathways in Actinomadura sp. ATCC 39365. Appl Environ Microbiol. 83(10). (*通讯作者)
15. Wu P#, Wan D#, Xu G #, Wang G, Ma H, Wang T, Gao Y, Qi J, Chen X, Zhu J, Li Y-Q, Deng Z, Chen W*. (2017) An unusual protector-protégé strategy for the biosynthesis of purine nucleoside antibiotics. Cell Chem Biol (Cell子刊). 24(2):171-181 (*通讯作者) (Selected as Best of Cell Chemical Biology in 2017)
16. He N#, Wu P#, Lei Y, Xu B, Zhu X, Xu G, Gao Y, Qi J, Deng Z, Tang G, Chen W*, Xiao Y*. (2017) Construction of the octosyl acid backbone catalyzed by a radical S-adenosylmethionine enzyme and a phosphatase in the biosynthesis of high-carbon sugar nucleoside antibiotics. Chem Sci. 8:444-451. (*共通讯作者)
17. Zhao H, Wang L, Wan D, Qi J, Gong R, Deng Z, Chen W*. (2016) Characterization of the aurantimycin biosynthetic gene cluster and enhancing its production by manipulating two pathway-specific activators in Streptomyces aurantiacus JA 4570. Microb Cell Fact. 15:160. (*通讯作者)
18. Qi J, Wan D, Ma H, Liu Y, Gong R, Qu X, Sun Y, Deng Z, Chen W*. (2016) Deciphering carbamoylpolyoxamic acid biosynthesis reveals unusual acetylation cycle associated with tandem reduction and sequential hydroxylation. Cell Chem Biol (Cell子刊). 23(8):935-944. (*通讯作者) (Featured as Issue Highlights in Cell Chem Biol)
19. Chen W#, Li Y#, Li J, Wu L, Li Y, Wang R, Deng Z, Zhou J. (2016) An unusual UMP C-5 methylase in nucleoside antibiotic polyoxin biosynthesis. Protein Cell. 7(9):673-683. (#共第一作者)
20. Chen W, Qi J, Wu P, Wan D, Liu J, Deng Z. (2016) Natural and engineered biosynthesis of nucleoside antibiotics in Actinomycetes. J Ind Microbiol Biotechnol. 43(2-3):401-17.
21. Qi J, Liu J, Wan D, Wang Y, Cai Y, Feng X, Wu P, Li S, Qiu G, Yang S, Chen W*, Deng Z. (2015) Metabolic engineering of an industrial polyoxin producer for the targeted overproduction of designer nucleoside antibiotics. Biotechnol Bioeng. 112(9):1865-1871. (*通讯作者) (Featured as Spotlight in Biotechol Bioeng)
22. Chen W, Dai D, Wang C, Huang T, Zhai L, Deng Z. (2013) Genetic dissection of the polyoxin building block-carbamoylpolyoxamic acid biosynthesis revealing the "pathway redundancy" in metabolic networks. Microb Cell Fact. 12:121.
23. Xu D, Liu G, Cheng L, Lu X, Chen W*, Deng Z*. (2013) Identification of Mur34 as the novel negative regulator responsible for the biosynthesis of muraymycin in Streptomyces sp. NRRL 30471. PLoS One. 8(10):e76068 (*共通讯作者)
24. Zhai L, Lin S, Qu D, Hong X, Bai L, Chen W*, Deng Z*. (2012) Engineering of an industrial polyoxin producer for the rational production of hybrid peptidyl nucleoside antibiotics. Metab Eng. 14(4):388-93. (*共通讯作者)
25. Doroghazi J, Ju K, Brown D, Labeda D, Deng Z, Metcalf W, Chen W*, Price NPJ*. (2011) Genome sequences of three tunicamycin-producing Streptomyces strains; S. chartreusis NRRL 12338, S. chartreusis NRRL 3882, and S. lysosuperificus ATCC 31396. J Bacteriol. 193(24):7021-2. (*共通讯作者)
26. Cheng L, Chen W*, Zhai L, Xu D, Huang T, Lin S, Zhou X, Deng Z*. (2011) Identification of the gene cluster involved in muraymycin biosynthesis from Streptomyces sp. NRRL 30471. Mol Biosyst. 7(3):920-927. (*共通讯作者)
27. Chen W#, Qu D#, Zhai L, Tao M, Wang Y, Lin S, Price NPJ, Deng Z. (2010) Characterization of the tunicamycin gene cluster unveiling unique steps involved in its biosynthesis. Protein Cell. 1(12):1093-1105. (#共第一作者)
28. Chen W, Huang T, He X, Meng Q, You D, Bai L, Li J, Wu M, Li R, Xie Z, Zhou H, Zhou X, Tan H, Deng Z. (2009) Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H. J Biol Chem. 284(16): 10627-10638.

1. 陈文青，龚蓉，邓子新，曾馨阅；多氧霉素杂合抗生素Polyoxin Ax和Polyoxin Hx及其制备方法和应用，申请号：202410926946.4。
2. 陈文青，吴叔文，王竹青，邓子新，张郑宇，李佳慧; 一种葡萄糖基化利巴韦林化合物及其制备方法与应用，申请号：202411501075.8。
3. 陈文青王逸飞一种依赖thyA基因互补而非抗生素的大肠杆菌质粒维持系统，申请号：202411324885.0。
4. 陈文青，于乐，邓子新，高耀杰，陈汝一，贺圆圆；高效生产5-甲基尿苷的高版本大肠杆菌基因工程菌及其应用，申请号：202410153359.6。
5. 陈文青, 于乐, 邓子新, 高耀杰, 刘洋, 陈汝一, 贺圆圆; 一种产5-甲基尿苷的优化基因工程菌及其应用, 申请号：202310946554.X。
6. 陈文青, 于乐, 邓子新, 陈汝一, 贺圆圆; 一种高效合成假尿苷的基因工程菌株及其应用, 申请号：202310946564.3。
7. 陈文青，于乐，邓子新，高耀杰，刘洋；一种生产5-甲基尿苷的基因工程菌及其应用，申请号：202210021004.2。
8. 陈文青，于乐，邓子新，周文婷，佘一玄；一种植物细胞分裂素狭霉素的生物合成基因簇及其生物材料以及在合成狭霉素中的应用，专利号：ZL202110689402.7。
9. 陈文青，孔丽媛，邓子新，徐顾丹，刘小琴，王静雯，唐曾琳；最小霉素生物合成基因簇、重组菌及其应用，专利号：ZL201911156223.6。
10. 陈文青，高耀杰，邓子新，徐顾丹，巫攀；2′-氯代喷司他丁和2′-氨基-2′-脱氧腺苷生物合成基因簇及其应用，专利号：ZL201710054193.2。
11. 陈文青，巫攀，邓子新，万丹，徐顾丹；喷司他丁和阿糖腺苷生物合成基因簇及其应用，专利号：ZL201611181302.9。
12. 陈文青，祁建钊，邓子新，刘进；肽基核苷类杂合抗生素Nikkoxin E和Nikkoxin F及其制备方法，专利号：ZL201510033114.0。
13. 陈文青，邓子新，翟李鹏，程放；多氧霉素与尼可霉素杂合抗生素及其制备方法，专利号：ZL201210049728.4。

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