精细化工

活性天然产物的合成生物学研究
挖掘和破解基因与天然产物合成之间的关联，是获取新型天然产物药物的关键。运用生物信息学与合成生物学的策略，解析天然产物的生物合成途径，为天然产物的绿色生物制造提供蓝图；进一步对微生物的代谢途径进行工程改造，促进具有高效生物活性的化合物或高附加值化工用品的微生物高效合成。
酶的催化机理研究和工程改造
运用结构生物学和酶工程等方法，研究酶催化反应的分子机制；对酶进行工程改造，实现更高的转化率、发现新的反应类型、构建天然产物衍生物的化合物库。

1) Hui Tao#; Lukas Lauterbach#; Guangkai Bian#; Rong Chen#; Anwei Hou#; Takahiro Mori#; Shu Cheng; Ben Hu; Li Lu; Xin Mu; Min Li; Naruhiko Adachi; Masato Kawasaki; Toshio Moriya; Toshiya Senda; Xinghuan Wang; Zixin Deng; Ikuro Abe*; Jeroen S Dickschat*; Tiangang Liu*. Discovery of non-squalene triterpenes. Nature, 2022, 606(7913): 414-419.
2) Hui Tao#, Richiro Uhimaru#,*, Takayoshi Awakawa, Takahiro Mori, Ikuro Abe*; Stereoselectivity and substrate specificity of the Fe (II)/α-ketoglutarate-dependent oxygenase TqaL. Journal of the American Chemical Society, 2022, 144(47): 21512-21520.
3) Hui Tao#; Takahiro Mori#,*; Heping Chen; Shuang Lyu; Akihito Nonoyama; Shoukou Lee; Ikuro Abe* ; Molecular insights into the unusually promiscuous and catalytically versatile Fe (II)/α-ketoglutarate-dependent oxygenase SptF, Nature Communications, 2022, 13(95).
4) Hui Tao; Takahiro Mori; Xingxing Wei; Yudai Matsuda*; Ikuro Abe*; One polyketide synthase, two distinct products: trans-acting enzyme-controlled product divergence in calbistrin biosynthesis, Angewandte Chemie International Edition, 2021, 60(16): 8851-8858.
5) Hui Tao; Ikuro Abe*; Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids. Current Opinion in Biotechnology, 2022, 77: 102763.
6) Hui Tao; Ikuro Abe*; Enzymology and biosynthesis of the orsellinic acid derived medicinal meroterpenoids, Current Opinion in Biotechnology, 2021, 69:52-59.
7) Hui Tao*; Ikuro Abe*; Oxidative modification of free-standing amino acids by Fe (II)/αKG-dependent oxygenases. Engineering Microbiology, 2022: 100062.
8) Ziheng An#; Hui Tao#; Yong Wang; Bingqing Xia; Yang Zou; Shuai Fu; Fang Fang; Xiao Sun; Renqiong Huang; Yao Xia; Zixin Deng; Ran Liu*; Tiangang Liu*; Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton, Synthetic and Systems Biotechnology, 2021, 6(4): 292-301.
9) Hui Tao#; Yuchen Zhang#; Zixin Deng; Tiangang Liu*; Strategies for enhancing the yield of the potent insecticide spinosad in actinomycetes, Biotechnology Journal, 2019, 14(1).
10) Hui Tao; Yuchen Zhang; Xiaoying Cao; Zixin Deng; Tiangang Liu*; Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification, Synthetic and Systems Biotechnology, 2016, 1(3): 150-157.
11) Hui Tao; Daoyi Guo; Yuchen Zhang; Zixin Deng; Tiangang Liu*; Metabolic engineering of microbes for branched-chain biodiesel production with low-temperature property, Biotechnology for Biofuels, 2015, 8(92).
12) Takahiro Mori#,*; Ziheng Yu#; Hui Tao; Ikuro Abe* ; Rational engineering of the nonheme iron-and 2-oxoglutarate-dependent oxygenase SptF, Organic Letters, 2022, 24(8): 1737-1741.
13) Tongxuan Bai#; Yudai Matsuda#,* ; Hui Tao; Takahiro Mori; Yonghui Zhang; Ikuro Abe* ; Structural diversification of andiconin-derived natural products by α-ketoglutarate-dependent dioxygenases, Organic Letters, 2020, 22(11): 4311-4315.
14) Gao-Yi Tan; Kunhua Deng; Xinhua Liu; Hui Tao; Yingying Chang; Jia Chen; Kai Chen; Zhi Sheng; Zixin Deng; Tiangang Liu*; Heterologous biosynthesis of spinosad: an omics-guided large polyketide synthase gene cluster reconstitution in streptomyces, ACS Synthetic Biology, 2017, 6(6): 995-1005.
15) Guangkai Bian; Yujie Yuan; Hui Tao; Xiaofei Shi; Xiaofang Zhong; Yichao Han; Shuai Fu; Chengxiang Fang; Zixin Deng; Tiangang Liu* ; Production of taxadiene by engineering of mevalonate pathway in Escherichia coli and endophytic fungus Alternaria alternata TPF6, Biotechnology Journal, 2017, 12(4).